Abstract
We present a large-area hemispherical perforated microwell structure for a bead based bioassay. The fabrication process for the hemispherical perforated microwell consisted of three steps: the convex micropatterned mold production from the concave micropatterned silicon wafer, hot embossing to generate the concave micropattened acrylate sheet, and reactive ion etching to make the bottom holes. We applied the hemispherical perforated microarray for the bead based aptamer screening, in which the bead trap, the in-situ fluorescence monitoring of aptamer-target protein interaction on the beads, the retrieval of the bioassay beads, and characterization of the aptamer by MALDI-TOF mass spectrometry could be sequentially executed.
| Original language | English |
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| Title of host publication | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 2312-2314 |
| Number of pages | 3 |
| ISBN (Electronic) | 9780979806476 |
| Publication status | Published - 2014 |
| Event | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States Duration: 26 Oct 2014 → 30 Oct 2014 |
Publication series
| Name | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 |
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Conference
| Conference | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 |
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| Country/Territory | United States |
| City | San Antonio |
| Period | 26/10/14 → 30/10/14 |
Bibliographical note
Publisher Copyright:© 14CBMS.
Keywords
- Aptamer screening
- Bead capture
- Bead microarray
- Bead retrieval
- Hemispherical perforated microwell