A new epicatechin gallate and calpain inhibitory activity from Orostachys japonicus

Hyoung Ja Kim; Jun Young Lee; Sung Min Kim; Da Ae Park; Changbae Jin; Seon Pyo Hong; Yong Sup Lee

doi:10.1016/j.fitote.2008.10.003

A new epicatechin gallate and calpain inhibitory activity from Orostachys japonicus

Hyoung Ja Kim
, Jun Young Lee
, Sung Min Kim
, Da Ae Park
, Changbae Jin
, Seon Pyo Hong
, Yong Sup Lee

Research output: Contribution to journal › Article › peer-review

38 Citations (Scopus)

Abstract

Calpains are calcium-dependent proteases that cleave a variety of intracellular substrates. The overactivation of μ-calpain is associated with a wide range of disease conditions. To search for calpain inhibitors from natural products, the phytochemical constituents of the ethyl acetate fraction of the whole plant of Orostachys japonicus (Crassulaceae) were studied. The various chromatographic separation of this fraction led to the isolation of a new tannin, (-)-epicatechin 5-gallate (1) along with 9 known compounds. Their structures were elucidated by spectroscopic and chemical analyses. Among them, (-)-epicatechin 5-gallate (1) and kaempferol (9) exhibited moderate inhibitory activity against μ-calpain with IC₅₀ values of 18.0 ± 2.9 and 15.4 ± 2.0 μg/ml, respectively.

Original language	English
Pages (from-to)	73-76
Number of pages	4
Journal	Fitoterapia
Volume	80
Issue number	1
DOIs	https://doi.org/10.1016/j.fitote.2008.10.003
Publication status	Published - Jan 2009

Bibliographical note

Funding Information:
This research was supported by the Mid-Term Technological Development Project funded by the Korean Ministry of Commerce, Industry and Energy (Grant No. 10027898-2007-22). S. M. Kim is grateful to the Ministry of Education & Human Resources Development, Korea for the support through the Post-BK21 Program.

Keywords

(-)-Epicatechin 5-gallate
Crassulaceae
Orostachys japonicus
μ-Calpain

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10.1016/j.fitote.2008.10.003

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title = "A new epicatechin gallate and calpain inhibitory activity from Orostachys japonicus",

abstract = "Calpains are calcium-dependent proteases that cleave a variety of intracellular substrates. The overactivation of μ-calpain is associated with a wide range of disease conditions. To search for calpain inhibitors from natural products, the phytochemical constituents of the ethyl acetate fraction of the whole plant of Orostachys japonicus (Crassulaceae) were studied. The various chromatographic separation of this fraction led to the isolation of a new tannin, (-)-epicatechin 5-gallate (1) along with 9 known compounds. Their structures were elucidated by spectroscopic and chemical analyses. Among them, (-)-epicatechin 5-gallate (1) and kaempferol (9) exhibited moderate inhibitory activity against μ-calpain with IC50 values of 18.0 ± 2.9 and 15.4 ± 2.0 μg/ml, respectively.",

keywords = "(-)-Epicatechin 5-gallate, Crassulaceae, Orostachys japonicus, μ-Calpain",

author = "Kim, \{Hyoung Ja\} and Lee, \{Jun Young\} and Kim, \{Sung Min\} and Park, \{Da Ae\} and Changbae Jin and Hong, \{Seon Pyo\} and Lee, \{Yong Sup\}",

note = "Funding Information: This research was supported by the Mid-Term Technological Development Project funded by the Korean Ministry of Commerce, Industry and Energy (Grant No. 10027898-2007-22). S. M. Kim is grateful to the Ministry of Education \& Human Resources Development, Korea for the support through the Post-BK21 Program.",

year = "2009",

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doi = "10.1016/j.fitote.2008.10.003",

language = "English",

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AU - Kim, Hyoung Ja

AU - Lee, Jun Young

AU - Kim, Sung Min

AU - Park, Da Ae

AU - Jin, Changbae

AU - Hong, Seon Pyo

AU - Lee, Yong Sup

N1 - Funding Information: This research was supported by the Mid-Term Technological Development Project funded by the Korean Ministry of Commerce, Industry and Energy (Grant No. 10027898-2007-22). S. M. Kim is grateful to the Ministry of Education & Human Resources Development, Korea for the support through the Post-BK21 Program.

PY - 2009/1

Y1 - 2009/1

N2 - Calpains are calcium-dependent proteases that cleave a variety of intracellular substrates. The overactivation of μ-calpain is associated with a wide range of disease conditions. To search for calpain inhibitors from natural products, the phytochemical constituents of the ethyl acetate fraction of the whole plant of Orostachys japonicus (Crassulaceae) were studied. The various chromatographic separation of this fraction led to the isolation of a new tannin, (-)-epicatechin 5-gallate (1) along with 9 known compounds. Their structures were elucidated by spectroscopic and chemical analyses. Among them, (-)-epicatechin 5-gallate (1) and kaempferol (9) exhibited moderate inhibitory activity against μ-calpain with IC50 values of 18.0 ± 2.9 and 15.4 ± 2.0 μg/ml, respectively.

AB - Calpains are calcium-dependent proteases that cleave a variety of intracellular substrates. The overactivation of μ-calpain is associated with a wide range of disease conditions. To search for calpain inhibitors from natural products, the phytochemical constituents of the ethyl acetate fraction of the whole plant of Orostachys japonicus (Crassulaceae) were studied. The various chromatographic separation of this fraction led to the isolation of a new tannin, (-)-epicatechin 5-gallate (1) along with 9 known compounds. Their structures were elucidated by spectroscopic and chemical analyses. Among them, (-)-epicatechin 5-gallate (1) and kaempferol (9) exhibited moderate inhibitory activity against μ-calpain with IC50 values of 18.0 ± 2.9 and 15.4 ± 2.0 μg/ml, respectively.

KW - (-)-Epicatechin 5-gallate

KW - Crassulaceae

KW - Orostachys japonicus

KW - μ-Calpain

UR - https://www.scopus.com/pages/publications/58149099943

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DO - 10.1016/j.fitote.2008.10.003

M3 - Article

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AN - SCOPUS:58149099943

SN - 0367-326X

VL - 80

SP - 73

EP - 76

JO - Fitoterapia

JF - Fitoterapia

IS - 1

ER -

A new epicatechin gallate and calpain inhibitory activity from Orostachys japonicus

Abstract

Bibliographical note

Keywords

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