A set of Saccharomyces cerevisiae integration vectors for fluorescent dye labeling of proteins

Inwha Baek; Sarah N. Le; Jongcheol Jeon; Yujin Chun; Charlotte Reed; Stephen Buratowski

doi:10.1093/g3journal/jkac201

A set of Saccharomyces cerevisiae integration vectors for fluorescent dye labeling of proteins

Inwha Baek, Sarah N. Le, Jongcheol Jeon, Yujin Chun, Charlotte Reed, Stephen Buratowski

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Protein fusions are frequently used for fluorescence imaging of individual molecules, both in vivo and in vitro. The SNAP, CLIP, HALO (aka HaloTag7), and DHFR protein tags can be linked to small molecule dyes that provide brightness and photo-stability superior to fluorescent proteins. To facilitate fluorescent dye tagging of proteins in the yeast Saccharomyces cerevisiae, we constructed a modular set of vectors with various combinations of labeling protein tags and selectable markers. These vectors can be used in combination to create strains where multiple proteins labeled with different colored dyes can be simultaneously observed.

Original language	English
Article number	jkac201
Journal	G3: Genes, Genomes, Genetics
Volume	12
Issue number	10
DOIs	https://doi.org/10.1093/g3journal/jkac201
Publication status	Published - Oct 2022

Bibliographical note

Publisher Copyright:
© The Author(s) 2022.

Keywords

CLIP
DHFR
HaloTag7
SNAP
Saccharomyces cerevisiae
epitope tagging
fluorescence microscopy
integration vector

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10.1093/g3journal/jkac201

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AU - Le, Sarah N.

AU - Jeon, Jongcheol

AU - Chun, Yujin

AU - Reed, Charlotte

AU - Buratowski, Stephen

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AB - Protein fusions are frequently used for fluorescence imaging of individual molecules, both in vivo and in vitro. The SNAP, CLIP, HALO (aka HaloTag7), and DHFR protein tags can be linked to small molecule dyes that provide brightness and photo-stability superior to fluorescent proteins. To facilitate fluorescent dye tagging of proteins in the yeast Saccharomyces cerevisiae, we constructed a modular set of vectors with various combinations of labeling protein tags and selectable markers. These vectors can be used in combination to create strains where multiple proteins labeled with different colored dyes can be simultaneously observed.

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A set of Saccharomyces cerevisiae integration vectors for fluorescent dye labeling of proteins

Abstract

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Keywords

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