The aim of the present study is to evaluate the bone morphogenic factor of PRF and PRP on rabbit calvaria. Twelve healthy rabbits, weighing 2.5∼3 kg, were used in this experiment. 8 mm diameter 4 hole were prepared on rabbit calvaria, each hole was filled with autologous bone, PRP and PRF. One defect site was filled with nothing as control group. According to experiment schedule, rabbits were sacrificed at 1, 2, and 6 weeks after surgery. Specimen were obtained using 10 mm trephin drill for real time RT-PCR and total calvaria were separated for H&E stain. RT-PCR was measured for mRNA expression of BMP-2, type I collagen, and osteopontin as a bone morphogenic factor. As a result, mRNA expression of BMP-2 in autologous bone group was higher than PRP and PRF group. PRP and PRF groups were higher than control group. In case of mRNA expression of Type I collagen, PRP and PRF groups progressively increased from 1 week to 6 weeks. In case of mRNA expression of OPN, PRF was higher at 2 weeks than 1 and 6 weeks. These result showed that PRP and PRF were more effective on the early stage of bone formation. H&E staining showed that autologous bone group was best. PRP and PRF group was better than control group. PRF showed similar effect comparing with PRP. From this, we can conclude that PRF can substitute for PRP.
|Number of pages||8|
|Journal||Tissue Engineering and Regenerative Medicine|
|Publication status||Published - Jun 2009|
- Real time RT-PCR
- Type I collagen