Absolute Quantification of Isoflavones in the Flowers of Pueraria lobata by qHNMR

Punam Thapa; Hye Mi Kim; Joon Pyo Hong; Ranhee Kim; Sunil Babu Paudel; Hyukjae Choi; Dae Sik Jang; Joo Won Nam

doi:10.3390/plants11040548

Absolute Quantification of Isoflavones in the Flowers of Pueraria lobata by qHNMR

Punam Thapa, Hye Mi Kim, Joon Pyo Hong, Ranhee Kim, Sunil Babu Paudel, Hyukjae Choi, Dae Sik Jang, Joo Won Nam

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

Pueraria lobata (Willd.) Ohwi. is a widely used medicinal plant in Korea, China, and Japan. The flower of P. lobata (Puerariae Flos) contains various bioactive substances such as triterpenoidal saponins and isoflavonoids. In this study, we developed a quantitative analysis of the isoflavones of Puerariae Flos by quantitative proton nuclear magnetic resonance (qHNMR) spectroscopy using the internal calibrant (IC). From the qHNMR results, the isoflavone content was found to be 7.99% and 10.57% for the MeOH sonication extract (PLs) and the MeOH reflux extract (PLr) of Puerariae Flos, respectively. The quantified isoflavone content was validated using the conventional analytical method, high-performance liquid chromatography with ultraviolet detection (HPLC-UV). The present study shows that validated qHNMR spectroscopy is a reliable method for quantifying and standardizing the isoflavone content in Puerariae Flos.

Original language	English
Article number	548
Journal	Plants
Volume	11
Issue number	4
DOIs	https://doi.org/10.3390/plants11040548
Publication status	Published - 1 Feb 2022

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Publisher Copyright:
© 2022 by the authors. Licensee MDPI, Basel, Switzerland.

Keywords

High-performance liquid chromatography (HPLC)
Isoflavone
Pueraria lobata
Puerariae Flos
Quantitative proton nuclear magnetic resonance (qHNMR)

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title = "Absolute Quantification of Isoflavones in the Flowers of Pueraria lobata by qHNMR",

abstract = "Pueraria lobata (Willd.) Ohwi. is a widely used medicinal plant in Korea, China, and Japan. The flower of P. lobata (Puerariae Flos) contains various bioactive substances such as triterpenoidal saponins and isoflavonoids. In this study, we developed a quantitative analysis of the isoflavones of Puerariae Flos by quantitative proton nuclear magnetic resonance (qHNMR) spectroscopy using the internal calibrant (IC). From the qHNMR results, the isoflavone content was found to be 7.99\% and 10.57\% for the MeOH sonication extract (PLs) and the MeOH reflux extract (PLr) of Puerariae Flos, respectively. The quantified isoflavone content was validated using the conventional analytical method, high-performance liquid chromatography with ultraviolet detection (HPLC-UV). The present study shows that validated qHNMR spectroscopy is a reliable method for quantifying and standardizing the isoflavone content in Puerariae Flos.",

keywords = "High-performance liquid chromatography (HPLC), Isoflavone, Pueraria lobata, Puerariae Flos, Quantitative proton nuclear magnetic resonance (qHNMR)",

author = "Punam Thapa and Kim, \{Hye Mi\} and Hong, \{Joon Pyo\} and Ranhee Kim and Paudel, \{Sunil Babu\} and Hyukjae Choi and Jang, \{Dae Sik\} and Nam, \{Joo Won\}",

note = "Publisher Copyright: {\textcopyright} 2022 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2022",

month = feb,

day = "1",

doi = "10.3390/plants11040548",

language = "English",

volume = "11",

journal = "Plants",

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T1 - Absolute Quantification of Isoflavones in the Flowers of Pueraria lobata by qHNMR

AU - Thapa, Punam

AU - Kim, Hye Mi

AU - Hong, Joon Pyo

AU - Kim, Ranhee

AU - Paudel, Sunil Babu

AU - Choi, Hyukjae

AU - Jang, Dae Sik

AU - Nam, Joo Won

PY - 2022/2/1

Y1 - 2022/2/1

N2 - Pueraria lobata (Willd.) Ohwi. is a widely used medicinal plant in Korea, China, and Japan. The flower of P. lobata (Puerariae Flos) contains various bioactive substances such as triterpenoidal saponins and isoflavonoids. In this study, we developed a quantitative analysis of the isoflavones of Puerariae Flos by quantitative proton nuclear magnetic resonance (qHNMR) spectroscopy using the internal calibrant (IC). From the qHNMR results, the isoflavone content was found to be 7.99% and 10.57% for the MeOH sonication extract (PLs) and the MeOH reflux extract (PLr) of Puerariae Flos, respectively. The quantified isoflavone content was validated using the conventional analytical method, high-performance liquid chromatography with ultraviolet detection (HPLC-UV). The present study shows that validated qHNMR spectroscopy is a reliable method for quantifying and standardizing the isoflavone content in Puerariae Flos.

AB - Pueraria lobata (Willd.) Ohwi. is a widely used medicinal plant in Korea, China, and Japan. The flower of P. lobata (Puerariae Flos) contains various bioactive substances such as triterpenoidal saponins and isoflavonoids. In this study, we developed a quantitative analysis of the isoflavones of Puerariae Flos by quantitative proton nuclear magnetic resonance (qHNMR) spectroscopy using the internal calibrant (IC). From the qHNMR results, the isoflavone content was found to be 7.99% and 10.57% for the MeOH sonication extract (PLs) and the MeOH reflux extract (PLr) of Puerariae Flos, respectively. The quantified isoflavone content was validated using the conventional analytical method, high-performance liquid chromatography with ultraviolet detection (HPLC-UV). The present study shows that validated qHNMR spectroscopy is a reliable method for quantifying and standardizing the isoflavone content in Puerariae Flos.

KW - High-performance liquid chromatography (HPLC)

KW - Isoflavone

KW - Pueraria lobata

KW - Puerariae Flos

KW - Quantitative proton nuclear magnetic resonance (qHNMR)

UR - https://www.scopus.com/pages/publications/85125560828

U2 - 10.3390/plants11040548

DO - 10.3390/plants11040548

M3 - Article

AN - SCOPUS:85125560828

SN - 2223-7747

VL - 11

JO - Plants

JF - Plants

IS - 4

M1 - 548

ER -

Absolute Quantification of Isoflavones in the Flowers of Pueraria lobata by qHNMR

Abstract

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Keywords

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