Alterations in grapevine leaf metabolism upon inoculation with Plasmopara viticola in different time-points

Kashif Ali; Federica Maltese; Andreia Figueiredo; Martina Rex; Ana Margarida Fortes; Eva Zyprian; Maria Salomé Pais; Robert Verpoorte; Young Hae Choi

doi:10.1016/j.plantsci.2012.04.014

Alterations in grapevine leaf metabolism upon inoculation with Plasmopara viticola in different time-points

Kashif Ali, Federica Maltese, Andreia Figueiredo, Martina Rex, Ana Margarida Fortes, Eva Zyprian, Maria Salomé Pais, Robert Verpoorte, Young Hae Choi

Research output: Contribution to journal › Article › peer-review

61 Citations (Scopus)

Abstract

Grapevines are easily infected by plant pathogens. It was found that resistant grapevines induce a wide range of phenolics upon the pathogen-infection. In this study in order to gain insight into these processes in different time-points the metabolic changes during the interaction of two grapevine cultivars, 'Regent' (resistant) and 'Trincadeira' (susceptible), with the downy mildew pathogen (Plasmopara viticola) were investigated. Nuclear magnetic resonance (NMR) spectroscopy on leaf extracts was used at several time points after experimental inoculation. A wide range of metabolites were identified using various two-dimensional (2D)-NMR techniques. Multivariate data analysis characterized both the resistant and the susceptible cultivars and their response against the pathogen. Metabolites responsible for their discrimination were identified as a fertaric acid, caftaric acid, quercetin-3-O-glucoside, linolenic acid, and alanine in the resistant cultivar 'Regent', while the susceptible 'Trincadeira' showed higher levels of glutamate, succinate, ascorbate and glucose. This study portrays the analytical capability of NMR spectroscopy and multivariate data analyses methods for the metabolic profiling of plant samples. The results obtained will underline the role of phenylpropanoids and flavonoids in resistance against biotic stresses which in turn provides a firm platform for the metabolic engineering of grapevine cultivars with higher resistance towards pathogens.

Original language	English
Pages (from-to)	100-107
Number of pages	8
Journal	Plant Science
Volume	191-192
DOIs	https://doi.org/10.1016/j.plantsci.2012.04.014
Publication status	Published - Aug 2012

Bibliographical note

Funding Information:
This work was supported by ERA-PG in Portugal and Germany under the project “Genomic Research-Assisted breeding for Sustainable Production of Quality GRAPEs and WINE” – GRASP ( http://www.erapg.org/everyone/9587/18624/18615/18661 ). The authors thank the Higher Education Commission (HEC) of Pakistan for the financial support of Kashif Ali.

Keywords

Grapevine
Metabolomics
Multivariate data analysis
NMR spectroscopy
Pathogen infection
Resistance

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10.1016/j.plantsci.2012.04.014

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@article{a6668b2f1bd74f1f8f15e91dce0722bf,

title = "Alterations in grapevine leaf metabolism upon inoculation with Plasmopara viticola in different time-points",

abstract = "Grapevines are easily infected by plant pathogens. It was found that resistant grapevines induce a wide range of phenolics upon the pathogen-infection. In this study in order to gain insight into these processes in different time-points the metabolic changes during the interaction of two grapevine cultivars, 'Regent' (resistant) and 'Trincadeira' (susceptible), with the downy mildew pathogen (Plasmopara viticola) were investigated. Nuclear magnetic resonance (NMR) spectroscopy on leaf extracts was used at several time points after experimental inoculation. A wide range of metabolites were identified using various two-dimensional (2D)-NMR techniques. Multivariate data analysis characterized both the resistant and the susceptible cultivars and their response against the pathogen. Metabolites responsible for their discrimination were identified as a fertaric acid, caftaric acid, quercetin-3-O-glucoside, linolenic acid, and alanine in the resistant cultivar 'Regent', while the susceptible 'Trincadeira' showed higher levels of glutamate, succinate, ascorbate and glucose. This study portrays the analytical capability of NMR spectroscopy and multivariate data analyses methods for the metabolic profiling of plant samples. The results obtained will underline the role of phenylpropanoids and flavonoids in resistance against biotic stresses which in turn provides a firm platform for the metabolic engineering of grapevine cultivars with higher resistance towards pathogens.",

keywords = "Grapevine, Metabolomics, Multivariate data analysis, NMR spectroscopy, Pathogen infection, Resistance",

author = "Kashif Ali and Federica Maltese and Andreia Figueiredo and Martina Rex and Fortes, {Ana Margarida} and Eva Zyprian and Pais, {Maria Salom{\'e}} and Robert Verpoorte and Choi, {Young Hae}",

note = "Funding Information: This work was supported by ERA-PG in Portugal and Germany under the project “Genomic Research-Assisted breeding for Sustainable Production of Quality GRAPEs and WINE” – GRASP ( http://www.erapg.org/everyone/9587/18624/18615/18661 ). The authors thank the Higher Education Commission (HEC) of Pakistan for the financial support of Kashif Ali.",

year = "2012",

month = aug,

doi = "10.1016/j.plantsci.2012.04.014",

language = "English",

volume = "191-192",

pages = "100--107",

journal = "Plant Science",

issn = "0168-9452",

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T1 - Alterations in grapevine leaf metabolism upon inoculation with Plasmopara viticola in different time-points

AU - Ali, Kashif

AU - Maltese, Federica

AU - Figueiredo, Andreia

AU - Rex, Martina

AU - Fortes, Ana Margarida

AU - Zyprian, Eva

AU - Pais, Maria Salomé

AU - Verpoorte, Robert

AU - Choi, Young Hae

N1 - Funding Information: This work was supported by ERA-PG in Portugal and Germany under the project “Genomic Research-Assisted breeding for Sustainable Production of Quality GRAPEs and WINE” – GRASP ( http://www.erapg.org/everyone/9587/18624/18615/18661 ). The authors thank the Higher Education Commission (HEC) of Pakistan for the financial support of Kashif Ali.

PY - 2012/8

Y1 - 2012/8

N2 - Grapevines are easily infected by plant pathogens. It was found that resistant grapevines induce a wide range of phenolics upon the pathogen-infection. In this study in order to gain insight into these processes in different time-points the metabolic changes during the interaction of two grapevine cultivars, 'Regent' (resistant) and 'Trincadeira' (susceptible), with the downy mildew pathogen (Plasmopara viticola) were investigated. Nuclear magnetic resonance (NMR) spectroscopy on leaf extracts was used at several time points after experimental inoculation. A wide range of metabolites were identified using various two-dimensional (2D)-NMR techniques. Multivariate data analysis characterized both the resistant and the susceptible cultivars and their response against the pathogen. Metabolites responsible for their discrimination were identified as a fertaric acid, caftaric acid, quercetin-3-O-glucoside, linolenic acid, and alanine in the resistant cultivar 'Regent', while the susceptible 'Trincadeira' showed higher levels of glutamate, succinate, ascorbate and glucose. This study portrays the analytical capability of NMR spectroscopy and multivariate data analyses methods for the metabolic profiling of plant samples. The results obtained will underline the role of phenylpropanoids and flavonoids in resistance against biotic stresses which in turn provides a firm platform for the metabolic engineering of grapevine cultivars with higher resistance towards pathogens.

AB - Grapevines are easily infected by plant pathogens. It was found that resistant grapevines induce a wide range of phenolics upon the pathogen-infection. In this study in order to gain insight into these processes in different time-points the metabolic changes during the interaction of two grapevine cultivars, 'Regent' (resistant) and 'Trincadeira' (susceptible), with the downy mildew pathogen (Plasmopara viticola) were investigated. Nuclear magnetic resonance (NMR) spectroscopy on leaf extracts was used at several time points after experimental inoculation. A wide range of metabolites were identified using various two-dimensional (2D)-NMR techniques. Multivariate data analysis characterized both the resistant and the susceptible cultivars and their response against the pathogen. Metabolites responsible for their discrimination were identified as a fertaric acid, caftaric acid, quercetin-3-O-glucoside, linolenic acid, and alanine in the resistant cultivar 'Regent', while the susceptible 'Trincadeira' showed higher levels of glutamate, succinate, ascorbate and glucose. This study portrays the analytical capability of NMR spectroscopy and multivariate data analyses methods for the metabolic profiling of plant samples. The results obtained will underline the role of phenylpropanoids and flavonoids in resistance against biotic stresses which in turn provides a firm platform for the metabolic engineering of grapevine cultivars with higher resistance towards pathogens.

KW - Grapevine

KW - Metabolomics

KW - Multivariate data analysis

KW - NMR spectroscopy

KW - Pathogen infection

KW - Resistance

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U2 - 10.1016/j.plantsci.2012.04.014

DO - 10.1016/j.plantsci.2012.04.014

M3 - Article

C2 - 22682569

AN - SCOPUS:84861420805

SN - 0168-9452

VL - 191-192

SP - 100

EP - 107

JO - Plant Science

JF - Plant Science

ER -

Alterations in grapevine leaf metabolism upon inoculation with Plasmopara viticola in different time-points

Abstract

Bibliographical note

Keywords

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