Amomum villosum induces longitudinal bone growth in adolescent female rats

Sun Haeng Lee, Ji Young Kim, Hocheol Kim, Seul Ki Park, Cho Young Kim, Sun Yong Chung, Gyu Tae Chang

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

OBJECTIVE: This study was conducted to evaluate the effect of Amomum villosum on longitudinal bone growth. METHODS: Adolescent female Sprague-Dawley rats were divided into 3 groups and treated for 4 days: control (distilled water, p.o.), recombinant human growth hormone (rhGH; 100 μg/kg, s.c.), and A. villosum (500 mg/kg, p.o.) groups. On day 3, tetracycline (20 g/kg, i.p.) was injected for growth plate identification. On days 2, 3 and 4, 5-bromo-2'-deoxyuridine (BrdU) (50 mg/kg, i.p.) was injected to label proliferating cells. On day 5, tibias were dissected and fixed in 4% paraformaldehyde, dehydrated, and sectioned for immunohistochemistry and histomorphometry. RESULTS: The rate of bone growth in the A. villosum and rhGH groups increased to (410 ± 44) and (389 ± 46) μm/day (P<0.01), respectively, as compared with the control (330.7 ± 34.7) μm/day. The thickness of the growth plates also increased to (591 ± 37) and (598 ± 32) μm, respectively, as compared with the control (524±89) μm (P<0.001). The number of BrdU-positive cells in the chondrocytes of the A. villosum and rhGH groups was also significantly higher (126±24) and (143±18) cells/mm2, respectively) than in the control (109 ± 25) mm2 (P<0.05). Insulin-like growth factor-1 and bone morphogenetic protein-2 in the A. villosum and rhGH groups were highly expressed in the growth plate as compared with the control samples, indicating increased bone formation. CONCLUSIONS: A. villosum could be used to treat growth retardation during adolescence.

Original languageEnglish
Pages (from-to)453-458
Number of pages6
JournalJournal of Traditional Chinese Medicine
Volume32
Issue number3
DOIs
Publication statusPublished - Sept 2012

Keywords

  • Amomum villosum
  • Bone Development
  • Growth plate
  • Recombinant human growth hormone

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