TY - JOUR
T1 - Amomum villosum induces longitudinal bone growth in adolescent female rats
AU - Lee, Sun Haeng
AU - Kim, Ji Young
AU - Kim, Hocheol
AU - Park, Seul Ki
AU - Kim, Cho Young
AU - Chung, Sun Yong
AU - Chang, Gyu Tae
PY - 2012/9
Y1 - 2012/9
N2 - OBJECTIVE: This study was conducted to evaluate the effect of Amomum villosum on longitudinal bone growth. METHODS: Adolescent female Sprague-Dawley rats were divided into 3 groups and treated for 4 days: control (distilled water, p.o.), recombinant human growth hormone (rhGH; 100 μg/kg, s.c.), and A. villosum (500 mg/kg, p.o.) groups. On day 3, tetracycline (20 g/kg, i.p.) was injected for growth plate identification. On days 2, 3 and 4, 5-bromo-2'-deoxyuridine (BrdU) (50 mg/kg, i.p.) was injected to label proliferating cells. On day 5, tibias were dissected and fixed in 4% paraformaldehyde, dehydrated, and sectioned for immunohistochemistry and histomorphometry. RESULTS: The rate of bone growth in the A. villosum and rhGH groups increased to (410 ± 44) and (389 ± 46) μm/day (P<0.01), respectively, as compared with the control (330.7 ± 34.7) μm/day. The thickness of the growth plates also increased to (591 ± 37) and (598 ± 32) μm, respectively, as compared with the control (524±89) μm (P<0.001). The number of BrdU-positive cells in the chondrocytes of the A. villosum and rhGH groups was also significantly higher (126±24) and (143±18) cells/mm2, respectively) than in the control (109 ± 25) mm2 (P<0.05). Insulin-like growth factor-1 and bone morphogenetic protein-2 in the A. villosum and rhGH groups were highly expressed in the growth plate as compared with the control samples, indicating increased bone formation. CONCLUSIONS: A. villosum could be used to treat growth retardation during adolescence.
AB - OBJECTIVE: This study was conducted to evaluate the effect of Amomum villosum on longitudinal bone growth. METHODS: Adolescent female Sprague-Dawley rats were divided into 3 groups and treated for 4 days: control (distilled water, p.o.), recombinant human growth hormone (rhGH; 100 μg/kg, s.c.), and A. villosum (500 mg/kg, p.o.) groups. On day 3, tetracycline (20 g/kg, i.p.) was injected for growth plate identification. On days 2, 3 and 4, 5-bromo-2'-deoxyuridine (BrdU) (50 mg/kg, i.p.) was injected to label proliferating cells. On day 5, tibias were dissected and fixed in 4% paraformaldehyde, dehydrated, and sectioned for immunohistochemistry and histomorphometry. RESULTS: The rate of bone growth in the A. villosum and rhGH groups increased to (410 ± 44) and (389 ± 46) μm/day (P<0.01), respectively, as compared with the control (330.7 ± 34.7) μm/day. The thickness of the growth plates also increased to (591 ± 37) and (598 ± 32) μm, respectively, as compared with the control (524±89) μm (P<0.001). The number of BrdU-positive cells in the chondrocytes of the A. villosum and rhGH groups was also significantly higher (126±24) and (143±18) cells/mm2, respectively) than in the control (109 ± 25) mm2 (P<0.05). Insulin-like growth factor-1 and bone morphogenetic protein-2 in the A. villosum and rhGH groups were highly expressed in the growth plate as compared with the control samples, indicating increased bone formation. CONCLUSIONS: A. villosum could be used to treat growth retardation during adolescence.
KW - Amomum villosum
KW - Bone Development
KW - Growth plate
KW - Recombinant human growth hormone
UR - http://www.scopus.com/inward/record.url?scp=84873458756&partnerID=8YFLogxK
U2 - 10.1016/s0254-6272(13)60054-0
DO - 10.1016/s0254-6272(13)60054-0
M3 - Article
C2 - 23297572
AN - SCOPUS:84873458756
SN - 0255-2922
VL - 32
SP - 453
EP - 458
JO - Journal of Traditional Chinese Medicine
JF - Journal of Traditional Chinese Medicine
IS - 3
ER -