Anti-allergic effects of myrciaria dubia (Camu-camu) fruit extract by inhibiting histamine h1 and h4 receptors and histidine decarboxylase in rbl-2h3 cells

Nhung Quynh Do, Shengdao Zheng, Sarang Oh, Quynh T.N. Nguyen, Minzhe Fang, Minseon Kim, Junhui Choi, Myeong Ju Kim, Jeehaeng Jeong, Tae Hoo Yi

Research output: Contribution to journalArticlepeer-review

Abstract

Although Myrciaria dubia (camu-camu) has been shown to exert anti-oxidant and antiinflammatory effects in both in vitro and in vivo studies, its use in allergic responses has not been elucidated. In the present study, the anti-allergic effect of 70% ethanol camu-camu fruit extract was tested on calcium ionophore (A23187)-induced allergies in RBL-2H3 cells. The RBL-2H3 cells were induced with 100 nM A23187 for 6 h, followed by a 1 h camu-camu fruit extract treatment. A23187 sanitization exacerbated mast cell degranulation; however, camu-camu fruit extract decreased the release of histamine and β-hexosaminidase, which are considered as key biomarkers in cell degranulation. Camu-camu fruit extract inhibited cell exocytosis by regulating the calcium/nuclear factor of activated T cell (NFAT) signaling. By downregulating the activation of mitogen-activated protein kinase (MAPK) signaling, camu-camu fruit extract hindered the activation of both histamine H1 and H4 receptors and inhibited histidine decarboxylase (HDC) expression by mediating its transcription factors KLF4/SP1 and GATA2/MITF. In A23187-induced ROS overproduction, camu-camu fruit extract activated nuclear factor erythroid-2-related factor 2 (Nrf2) to protect mast cells against A23187-induced oxidative stress. These findings indicate that camu-camu fruit extract can be developed to act as a mast cell stabilizer and an anti-histamine. This work also “opens the door” to new investigations using natural products to achieve breakthroughs in allergic disorder treatment.

Original languageEnglish
Article number104
JournalAntioxidants
Volume11
Issue number1
DOIs
Publication statusPublished - Jan 2022

Keywords

  • Anti-allergic effect
  • Camu-camu fruit extract
  • Histamine H1 receptor
  • Histamine H4 receptor
  • Histidine decarboxylase
  • RBL-2H3 cells

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