Anti-cancer effect of bee venom on human MDA-MB-231 breast cancer cells using raman spectroscopy

Gyeong Bok Jung, Jeong Eun Huh, Hyo Jung Lee, Dohyun Kim, Gi Ja Lee, Hun Kuk Park, Jae Dong Lee

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

We demonstrated the apoptotic effect of bee venom (BV) on human MDA-MB-231 breast cancer cells using Raman spectroscopy and principal component analysis (PCA). Biochemical changes in cancer cells were monitored following BV treatment; the results for different concentrations and treatment durations differed markedly. Significantly decreased Raman vibrations for DNA and proteins were observed for cells treated with 3.0 µg/mL BV for 48 h compared with those of control cells. These results suggest denaturation and degradation of proteins and DNA fragmentation (all cell death-related processes). The Raman spectroscopy results agreed with those of atomic force microscopy and conventional biological tests such as viability, TUNEL, and western blot assays. Therefore, Raman spectroscopy, with PCA, provides a noninvasive, label-free tool for assessment of cellular changes on the anti-cancer effect of BV.

Original languageEnglish
Article number#345244
Pages (from-to)5703-5718
Number of pages16
JournalBiomedical Optics Express
Volume9
Issue number11
DOIs
Publication statusPublished - 1 Nov 2018

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