Anti-nephrolithic potential of resveratrol via inhibition of ROS, MCP-1, hyaluronan and osteopontin in vitro and in vivo

Sang Hyuk Hong; Hyo Jung Lee; Eun Jung Sohn; Hyun Suk Ko; Bum Sang Shim; Kyoo Seok Ahn; Sung Hoon Kim

doi:10.1016/s1734-1140(13)71078-8

Anti-nephrolithic potential of resveratrol via inhibition of ROS, MCP-1, hyaluronan and osteopontin in vitro and in vivo

Sang Hyuk Hong
, Hyo Jung Lee
, Eun Jung Sohn
, Hyun Suk Ko
, Bum Sang Shim
, Kyoo Seok Ahn
, Sung Hoon Kim

Research output: Contribution to journal › Article › peer-review

48 Citations (Scopus)

Abstract

Background: Though resveratrol is known to have anti-cancer, anti-diabetic, anti-oxidant and anti-inflammatory activities, The inhibitory mechanism of resveratrol in kidney stone formation has not been elucidated so far. Method: ELISA, flow cytometry, RT-PCR, and western blotting were performed. Human renal epiThelial cells (HRCs) and rats with ethylene glycol (EG)-induced kidney stones were used. Results: Awound healing assay revealed that resveratrol significantly inhibited The oxalate-mediated migration of HRCs, considering oxalate mediates kidney stone formation. Also, resveratrol suppressed The mRNAexpression of nicotinamide adenine dinucleotide phosphate hydrogen (NADPH) oxidase subunits such as p22phox and p47phox, monocyte chemoattractant protein 1 (MCP-1) and osteopontin (OPN) in oxalate-treated HRCs. FurThermore, western blotting showed that resveratrol downregulated The expression of MCP-1-related proteins including transforming growth factor(TGF-b1), TGFR-I or II and hyaluronan in oxalate-treated HRCs. Consistently, resveratrol reduced oxalate-mediated production of reactive oxygen species (ROS) and malondialdehyde (MDA) in oxalate-treated HRCs, while The activities of anti-oxidant enzymes such as superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) were enhanced by resveratrol in HRCs and EG-treated kidneys of rats. Consistently, resveratrol significantly reduced The number of urine calcium oxalate crystals and serum MDA, and attenuated The expression of OPN and hyaluroran in EG-treated rats. Conclusions: Our findings suggest that resveratrol exerts anti-nephrolithic potential via inhibition of ROS, MCP-1 hyaluronan and OPN signaling.

Original language	English
Pages (from-to)	970-979
Number of pages	10
Journal	Pharmacological Reports
Volume	65
Issue number	4
DOIs	https://doi.org/10.1016/s1734-1140(13)71078-8
Publication status	Published - 2013

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation of Korea (NRF) grant (No. 2011-0017956 ) and Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea government [ MEST ] (No. 2012-0005755 ).

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Hyaluronan
Mcp-1
Opn
Oxalate
Resveratrol
Ros

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10.1016/s1734-1140(13)71078-8

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@article{9de4a9622f6a495e80ea072e88b43760,

title = "Anti-nephrolithic potential of resveratrol via inhibition of ROS, MCP-1, hyaluronan and osteopontin in vitro and in vivo",

abstract = "Background: Though resveratrol is known to have anti-cancer, anti-diabetic, anti-oxidant and anti-inflammatory activities, The inhibitory mechanism of resveratrol in kidney stone formation has not been elucidated so far. Method: ELISA, flow cytometry, RT-PCR, and western blotting were performed. Human renal epiThelial cells (HRCs) and rats with ethylene glycol (EG)-induced kidney stones were used. Results: Awound healing assay revealed that resveratrol significantly inhibited The oxalate-mediated migration of HRCs, considering oxalate mediates kidney stone formation. Also, resveratrol suppressed The mRNAexpression of nicotinamide adenine dinucleotide phosphate hydrogen (NADPH) oxidase subunits such as p22phox and p47phox, monocyte chemoattractant protein 1 (MCP-1) and osteopontin (OPN) in oxalate-treated HRCs. FurThermore, western blotting showed that resveratrol downregulated The expression of MCP-1-related proteins including transforming growth factor(TGF-b1), TGFR-I or II and hyaluronan in oxalate-treated HRCs. Consistently, resveratrol reduced oxalate-mediated production of reactive oxygen species (ROS) and malondialdehyde (MDA) in oxalate-treated HRCs, while The activities of anti-oxidant enzymes such as superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) were enhanced by resveratrol in HRCs and EG-treated kidneys of rats. Consistently, resveratrol significantly reduced The number of urine calcium oxalate crystals and serum MDA, and attenuated The expression of OPN and hyaluroran in EG-treated rats. Conclusions: Our findings suggest that resveratrol exerts anti-nephrolithic potential via inhibition of ROS, MCP-1 hyaluronan and OPN signaling.",

keywords = "Hyaluronan, Mcp-1, Opn, Oxalate, Resveratrol, Ros",

author = "Hong, \{Sang Hyuk\} and Lee, \{Hyo Jung\} and Sohn, \{Eun Jung\} and Ko, \{Hyun Suk\} and Shim, \{Bum Sang\} and Ahn, \{Kyoo Seok\} and Kim, \{Sung Hoon\}",

note = "Funding Information: This work was supported by the National Research Foundation of Korea (NRF) grant (No. 2011-0017956 ) and Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea government [ MEST ] (No. 2012-0005755 ).",

year = "2013",

doi = "10.1016/s1734-1140(13)71078-8",

language = "English",

volume = "65",

pages = "970--979",

journal = "Pharmacological Reports",

issn = "1734-1140",

publisher = "Springer Science and Business Media Deutschland GmbH",

number = "4",

}

TY - JOUR

T1 - Anti-nephrolithic potential of resveratrol via inhibition of ROS, MCP-1, hyaluronan and osteopontin in vitro and in vivo

AU - Hong, Sang Hyuk

AU - Lee, Hyo Jung

AU - Sohn, Eun Jung

AU - Ko, Hyun Suk

AU - Shim, Bum Sang

AU - Ahn, Kyoo Seok

AU - Kim, Sung Hoon

N1 - Funding Information: This work was supported by the National Research Foundation of Korea (NRF) grant (No. 2011-0017956 ) and Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea government [ MEST ] (No. 2012-0005755 ).

PY - 2013

Y1 - 2013

N2 - Background: Though resveratrol is known to have anti-cancer, anti-diabetic, anti-oxidant and anti-inflammatory activities, The inhibitory mechanism of resveratrol in kidney stone formation has not been elucidated so far. Method: ELISA, flow cytometry, RT-PCR, and western blotting were performed. Human renal epiThelial cells (HRCs) and rats with ethylene glycol (EG)-induced kidney stones were used. Results: Awound healing assay revealed that resveratrol significantly inhibited The oxalate-mediated migration of HRCs, considering oxalate mediates kidney stone formation. Also, resveratrol suppressed The mRNAexpression of nicotinamide adenine dinucleotide phosphate hydrogen (NADPH) oxidase subunits such as p22phox and p47phox, monocyte chemoattractant protein 1 (MCP-1) and osteopontin (OPN) in oxalate-treated HRCs. FurThermore, western blotting showed that resveratrol downregulated The expression of MCP-1-related proteins including transforming growth factor(TGF-b1), TGFR-I or II and hyaluronan in oxalate-treated HRCs. Consistently, resveratrol reduced oxalate-mediated production of reactive oxygen species (ROS) and malondialdehyde (MDA) in oxalate-treated HRCs, while The activities of anti-oxidant enzymes such as superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) were enhanced by resveratrol in HRCs and EG-treated kidneys of rats. Consistently, resveratrol significantly reduced The number of urine calcium oxalate crystals and serum MDA, and attenuated The expression of OPN and hyaluroran in EG-treated rats. Conclusions: Our findings suggest that resveratrol exerts anti-nephrolithic potential via inhibition of ROS, MCP-1 hyaluronan and OPN signaling.

AB - Background: Though resveratrol is known to have anti-cancer, anti-diabetic, anti-oxidant and anti-inflammatory activities, The inhibitory mechanism of resveratrol in kidney stone formation has not been elucidated so far. Method: ELISA, flow cytometry, RT-PCR, and western blotting were performed. Human renal epiThelial cells (HRCs) and rats with ethylene glycol (EG)-induced kidney stones were used. Results: Awound healing assay revealed that resveratrol significantly inhibited The oxalate-mediated migration of HRCs, considering oxalate mediates kidney stone formation. Also, resveratrol suppressed The mRNAexpression of nicotinamide adenine dinucleotide phosphate hydrogen (NADPH) oxidase subunits such as p22phox and p47phox, monocyte chemoattractant protein 1 (MCP-1) and osteopontin (OPN) in oxalate-treated HRCs. FurThermore, western blotting showed that resveratrol downregulated The expression of MCP-1-related proteins including transforming growth factor(TGF-b1), TGFR-I or II and hyaluronan in oxalate-treated HRCs. Consistently, resveratrol reduced oxalate-mediated production of reactive oxygen species (ROS) and malondialdehyde (MDA) in oxalate-treated HRCs, while The activities of anti-oxidant enzymes such as superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) were enhanced by resveratrol in HRCs and EG-treated kidneys of rats. Consistently, resveratrol significantly reduced The number of urine calcium oxalate crystals and serum MDA, and attenuated The expression of OPN and hyaluroran in EG-treated rats. Conclusions: Our findings suggest that resveratrol exerts anti-nephrolithic potential via inhibition of ROS, MCP-1 hyaluronan and OPN signaling.

KW - Hyaluronan

KW - Mcp-1

KW - Opn

KW - Oxalate

KW - Resveratrol

KW - Ros

UR - https://www.scopus.com/pages/publications/84886458326

U2 - 10.1016/s1734-1140(13)71078-8

DO - 10.1016/s1734-1140(13)71078-8

M3 - Article

C2 - 24145091

AN - SCOPUS:84886458326

SN - 1734-1140

VL - 65

SP - 970

EP - 979

JO - Pharmacological Reports

JF - Pharmacological Reports

IS - 4

ER -

Anti-nephrolithic potential of resveratrol via inhibition of ROS, MCP-1, hyaluronan and osteopontin in vitro and in vivo

Abstract

Bibliographical note

UN SDGs

Keywords

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