Abstract
Aim of the study: This study evaluated anti-obesity effect of the ethanolic extract of Morus bombycis root on lipase activity and lipolysis in adipocytes and adipose tissues. Materials and methods: Lipase (triacylgycerol acylhydrolase, EC 3.1.1.3) activity was determined by measuring the hydrolysis of p-nitrophenyl butyrate to p-nitrophenol at 405. nm. Lipolytic effects were assayed in fully differentiated 3T3-L1 adipocytes and adipose tissues. In vitro, phosphodiesterase (PDE) activity was also measured. Results: Morus bombycis root extract exhibited strong anti-lipase activity, with an IC50 value of 2.07μg/mL. In differentiated adipocytes and adipose tissues, the extract increased lipolytic effects such as decreased intracellular triglyceride and the release of glycerol. Further, the extract inhibited PDE activity in a dose-dependent manner. Conclusion: The present study suggests that Morus bombycis root extract might be of therapeutic interest with respect to the treatment of obesity.
| Original language | English |
|---|---|
| Pages (from-to) | 621-624 |
| Number of pages | 4 |
| Journal | Journal of Ethnopharmacology |
| Volume | 130 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Aug 2010 |
Keywords
- Anti-lipase activity
- Lipolytic effects
- Morus bombycis
- Phosphodiesterase
- Traditional Korean herbal medicines
