Antioxidant capacity of 12 major soybean isoflavones and their bioavailability under simulated digestion and in human intestinal Caco-2 cells

Mi Seon Kim, Young Sung Jung, Davin Jang, Chi Heung Cho, Sang Hoon Lee, Nam Soo Han, Dae Ok Kim

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Soy isoflavones (SIs) show various health benefits, such as antioxidant and estrogenic effects. It is important to understand the bioaccessibility and bioavailability of SIs due to the close relation to their bioactivities. In this study, the antioxidant capacity, bioaccessibility, and bioavailability of 12 SIs were evaluated using radical-scavenging methods, simulations of human digestion, and Caco-2 cells in Transwell, respectively. All SIs were stable (91.1–99.2%) under gastric digestion conditions compared with the control (100%), whereas acetyl and malonyl conjugates were unstable (38.5% and 65.5%, respectively) under small intestinal digestion conditions. SI aglycones showed higher permeability (7–15 times) and cellular accumulation (8.8 times) than their glucosides. A small amount of SI conjugates was intact in the cell and in the basolateral side of each Transwell. These results suggest that SI conjugates, especially malonyl and acetyl forms, have incidental bioactivity after being metabolized to aglycones inside the cell.

Original languageEnglish
Article number131493
JournalFood Chemistry
Volume374
DOIs
Publication statusPublished - 16 Apr 2022

Keywords

  • Bioaccessibility
  • Cellular accumulation
  • Digestion stability
  • Isoflavone conjugate
  • Permeability
  • Transwell system

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