Apoptosis signal-regulating kinase 1 regulates the expression of caspase-11

Jong Ryoul Choi; Hyejung Heo; Yoo Lang; Ki Soon Shin; Shin Jung Kang

doi:10.1016/j.febslet.2009.08.014

Apoptosis signal-regulating kinase 1 regulates the expression of caspase-11

Jong Ryoul Choi, Hyejung Heo, Yoo Lang, Ki Soon Shin, Shin Jung Kang

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Caspase-11 is an inducible caspase involved in the regulation of cell death and inflammation. In the present study, we examined whether apoptosis signal-regulating kinase 1 (Ask1)-mediated signaling pathway is involved in the expression of caspase-11 induced by lipopolysaccharide (LPS). We found that the induction of caspase-11 was suppressed by the inhibitors of NADPH oxidase (Nox) or knockdown of Nox4 that acts downstream of toll-like receptor 4 and generates Ask1-activating reactive oxygen species. Overexpression of dominant negative tumor necrosis factor receptor associate factor 6 also suppressed the induction of caspase-11. Importantly, knockdown or dominant negative form of Ask1 suppressed the induction of caspase-11 following LPS stimulation. Taken together, our results show that Ask1 regulates the expression of caspase-11 following LPS stimulation.

Original language	English
Pages (from-to)	3016-3020
Number of pages	5
Journal	FEBS Letters
Volume	583
Issue number	18
DOIs	https://doi.org/10.1016/j.febslet.2009.08.014
Publication status	Published - 17 Sept 2009

Bibliographical note

Funding Information:
This work was supported by a grant from the Korea Research Foundation ( KRF-2006-312-C00284 ) and a grant from the Korea Science and Engineering Foundation ( R01-2007-000-21015-0 ). We thank Dr. H. Ichijo for generously providing Ask1 expression vectors, Dr. E. Choi for the TRAF6 expression vectors, and Dr. R. Beyaert for the caspase-11 promoter-luciferase expression vectors.

Keywords

Ask1
Caspase-11
LPS
Nox4
TLR4

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10.1016/j.febslet.2009.08.014

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title = "Apoptosis signal-regulating kinase 1 regulates the expression of caspase-11",

abstract = "Caspase-11 is an inducible caspase involved in the regulation of cell death and inflammation. In the present study, we examined whether apoptosis signal-regulating kinase 1 (Ask1)-mediated signaling pathway is involved in the expression of caspase-11 induced by lipopolysaccharide (LPS). We found that the induction of caspase-11 was suppressed by the inhibitors of NADPH oxidase (Nox) or knockdown of Nox4 that acts downstream of toll-like receptor 4 and generates Ask1-activating reactive oxygen species. Overexpression of dominant negative tumor necrosis factor receptor associate factor 6 also suppressed the induction of caspase-11. Importantly, knockdown or dominant negative form of Ask1 suppressed the induction of caspase-11 following LPS stimulation. Taken together, our results show that Ask1 regulates the expression of caspase-11 following LPS stimulation.",

keywords = "Ask1, Caspase-11, LPS, Nox4, TLR4",

author = "Choi, {Jong Ryoul} and Hyejung Heo and Yoo Lang and Shin, {Ki Soon} and Kang, {Shin Jung}",

note = "Funding Information: This work was supported by a grant from the Korea Research Foundation ( KRF-2006-312-C00284 ) and a grant from the Korea Science and Engineering Foundation ( R01-2007-000-21015-0 ). We thank Dr. H. Ichijo for generously providing Ask1 expression vectors, Dr. E. Choi for the TRAF6 expression vectors, and Dr. R. Beyaert for the caspase-11 promoter-luciferase expression vectors.",

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doi = "10.1016/j.febslet.2009.08.014",

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AU - Lang, Yoo

AU - Shin, Ki Soon

AU - Kang, Shin Jung

N1 - Funding Information: This work was supported by a grant from the Korea Research Foundation ( KRF-2006-312-C00284 ) and a grant from the Korea Science and Engineering Foundation ( R01-2007-000-21015-0 ). We thank Dr. H. Ichijo for generously providing Ask1 expression vectors, Dr. E. Choi for the TRAF6 expression vectors, and Dr. R. Beyaert for the caspase-11 promoter-luciferase expression vectors.

PY - 2009/9/17

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N2 - Caspase-11 is an inducible caspase involved in the regulation of cell death and inflammation. In the present study, we examined whether apoptosis signal-regulating kinase 1 (Ask1)-mediated signaling pathway is involved in the expression of caspase-11 induced by lipopolysaccharide (LPS). We found that the induction of caspase-11 was suppressed by the inhibitors of NADPH oxidase (Nox) or knockdown of Nox4 that acts downstream of toll-like receptor 4 and generates Ask1-activating reactive oxygen species. Overexpression of dominant negative tumor necrosis factor receptor associate factor 6 also suppressed the induction of caspase-11. Importantly, knockdown or dominant negative form of Ask1 suppressed the induction of caspase-11 following LPS stimulation. Taken together, our results show that Ask1 regulates the expression of caspase-11 following LPS stimulation.

AB - Caspase-11 is an inducible caspase involved in the regulation of cell death and inflammation. In the present study, we examined whether apoptosis signal-regulating kinase 1 (Ask1)-mediated signaling pathway is involved in the expression of caspase-11 induced by lipopolysaccharide (LPS). We found that the induction of caspase-11 was suppressed by the inhibitors of NADPH oxidase (Nox) or knockdown of Nox4 that acts downstream of toll-like receptor 4 and generates Ask1-activating reactive oxygen species. Overexpression of dominant negative tumor necrosis factor receptor associate factor 6 also suppressed the induction of caspase-11. Importantly, knockdown or dominant negative form of Ask1 suppressed the induction of caspase-11 following LPS stimulation. Taken together, our results show that Ask1 regulates the expression of caspase-11 following LPS stimulation.

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Apoptosis signal-regulating kinase 1 regulates the expression of caspase-11

Abstract

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Keywords

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