Aurones and flavonols from coreopsis lanceolata l. Flowers and their anti-oxidant, pro-inflammatory inhibition effects, and recovery effects on alloxan-induced pancreatic islets in zebrafish

Hyoung Geun Kim, Youn Hee Nam, Young Sung Jung, Seon Min Oh, Trong Nguyen Nguyen, Min Ho Lee, Dae Ok Kim, Tong Ho Kang, Dae Young Lee, Nam In Baek

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

(1) Background: Many flavonoids have been reported to exhibit pharmacological activity; a preparatory study confirmed that Coreopsis lanceolata flowers (CLFs) contained high flavonoid structure content; (2) Methods: CLFs were extracted in aqueous methanol (MeOH:H2O = 4:1) and fractionated into acetic ester (EtOAc), normal butanol (n-BuOH), and H2O fractions. Repeated column chromatographies for two fractions led to the isolation of two aurones and two flavonols; (3) Results: Four flavonoids were identified based on a variety of spectroscopic data analyses to be leptosidin (1), leptosin (2), isoquercetin (3), and astragalin (4), respectively. This is the first report for isolation of 2–4 from CLFs. High-performance liquid chromatography (HPLC) analysis determined the content levels of compounds 1–4 in the MeOH extract to be 2.8 ± 0.3 mg/g (1), 17.9 ± 0.9 mg/g (2), 3.0 ± 0.2 mg/g (3), and 10.9 ± 0.9 mg/g (4), respectively. All isolated compounds showed radical scavenging activities and recovery activities in Caco-2, RAW264.7, PC-12, and HepG2 cells against reactive oxygen species. MeOH extract, EtOAc fraction, and 1–3 suppressed NO formation in LPS-stimulated RAW 264.7 cells and decreased iNOS and COX-2 expression. Furthermore, all compounds recovered the pancreatic islets damaged by alloxan treatment in zebrafish; (4) Conclusions: The out-come proposes 1–4 to serve as components of CLFs in standardizing anti-oxidant, pro-inflammatory inhibition, and potential anti-diabetic agents.

Original languageEnglish
Article number6098
JournalMolecules
Volume26
Issue number20
DOIs
Publication statusPublished - 1 Oct 2021

Keywords

  • Anti-inflammation
  • Antioxidant capacity
  • Coreopsis lanceolata
  • Flavonoid
  • Pancreatic islets
  • RAW 264.7
  • Zebrafish

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