TY - GEN
T1 - Automated microfluidic system for RNA purification using a centrifugal force
AU - Park, Byung Hyun
AU - Jung, Jae Hwan
AU - Zhang, Hainan
AU - Lee, Nae Yoon
AU - Seo, Tae Seok
N1 - Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 2012
Y1 - 2012
N2 - Herein, we demonstrated a simple, rapid and automated RNA purification system based on a centrifugal force using three passive capillary valves and a silica sol-gel matrix. We designed three reservoirs (sample, washing solution, elution buffer) which were connected with different dimensional microfluidic channels. By controlling RPM, we could dispense the RNA sample, washing solution, and elution buffer successively, so that the RNA was captured in the sol-gel solid phase, purified, and eluted. This novel rotary sample preparation system eliminates any complicated hardware and human intervention, and performs the RNA preparation with high speed and portability.
AB - Herein, we demonstrated a simple, rapid and automated RNA purification system based on a centrifugal force using three passive capillary valves and a silica sol-gel matrix. We designed three reservoirs (sample, washing solution, elution buffer) which were connected with different dimensional microfluidic channels. By controlling RPM, we could dispense the RNA sample, washing solution, and elution buffer successively, so that the RNA was captured in the sol-gel solid phase, purified, and eluted. This novel rotary sample preparation system eliminates any complicated hardware and human intervention, and performs the RNA preparation with high speed and portability.
KW - Centrifugal force
KW - Nucleic acid purification
KW - RPM control
UR - http://www.scopus.com/inward/record.url?scp=84901774184&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84901774184
SN - 9780979806452
T3 - Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
SP - 1174
EP - 1176
BT - Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PB - Chemical and Biological Microsystems Society
T2 - 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
Y2 - 28 October 2012 through 1 November 2012
ER -