Biochemical analysis of BmNPV attachment to Bombyx Mori BmN-4 cells

Tae Yong Kim; Han Chul Kang; Jin O. Park; Jong Hwa Park; Seok Kwon Kang; In Sik Chung

doi:10.1007/BF02932465

Biochemical analysis of BmNPV attachment to Bombyx Mori BmN-4 cells

Tae Yong Kim
, Han Chul Kang
, Jin O. Park
, Jong Hwa Park
, Seok Kwon Kang
, In Sik Chung

Research output: Contribution to journal › Article › peer-review

Abstract

Binding characteristics of Bombyx mori nuclear polyhedrosis virus (BmNPV) to B. mori BmN-4 cells was determined. The cells had a single class of BmNPV-binding sites and displayed a low binding capacity for BmNPV in the range of 70 sites per cell. The biochemical nature of BmNPV-binding sites on the cell surface was identified. Treatment of BmN-4 cells with protease, wheat germ agglutinin, metabolic inhibitors, or lysosomotropic agents reduced BmNPV binding to BmN-4 cells, whereas treatment with phospholipase C showed no effect on virus binding. These results indicate that the structure of the binding site moiety is a protein-oligosaccharide complex.

Original language	English
Pages (from-to)	59-61
Number of pages	3
Journal	Biotechnology and Bioprocess Engineering
Volume	2
Issue number	1
DOIs	https://doi.org/10.1007/BF02932465
Publication status	Published - 1997

Bibliographical note

Funding Information:
Acknowledgment The present studies were supported by the Basic Science Research Institute Program, Ministry of Education, 1995, Project No.

Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.

Keywords

Binding site
BmN-4 cells
Bombyx mori nuclear polyhedrosis virus

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10.1007/BF02932465

Cite this

@article{cafb5d7b20c54b14935f042217239adf,

title = "Biochemical analysis of BmNPV attachment to Bombyx Mori BmN-4 cells",

abstract = "Binding characteristics of Bombyx mori nuclear polyhedrosis virus (BmNPV) to B. mori BmN-4 cells was determined. The cells had a single class of BmNPV-binding sites and displayed a low binding capacity for BmNPV in the range of 70 sites per cell. The biochemical nature of BmNPV-binding sites on the cell surface was identified. Treatment of BmN-4 cells with protease, wheat germ agglutinin, metabolic inhibitors, or lysosomotropic agents reduced BmNPV binding to BmN-4 cells, whereas treatment with phospholipase C showed no effect on virus binding. These results indicate that the structure of the binding site moiety is a protein-oligosaccharide complex.",

keywords = "Binding site, BmN-4 cells, Bombyx mori nuclear polyhedrosis virus",

author = "Kim, \{Tae Yong\} and Kang, \{Han Chul\} and Park, \{Jin O.\} and Park, \{Jong Hwa\} and Kang, \{Seok Kwon\} and Chung, \{In Sik\}",

note = "Funding Information: Acknowledgment The present studies were supported by the Basic Science Research Institute Program, Ministry of Education, 1995, Project No. Copyright: Copyright 2018 Elsevier B.V., All rights reserved.",

year = "1997",

doi = "10.1007/BF02932465",

language = "English",

volume = "2",

pages = "59--61",

journal = "Biotechnology and Bioprocess Engineering",

issn = "1226-8372",

publisher = "Korean Society for Biotechnology and Bioengineering",

number = "1",

}

TY - JOUR

T1 - Biochemical analysis of BmNPV attachment to Bombyx Mori BmN-4 cells

AU - Kim, Tae Yong

AU - Kang, Han Chul

AU - Park, Jin O.

AU - Park, Jong Hwa

AU - Kang, Seok Kwon

AU - Chung, In Sik

N1 - Funding Information: Acknowledgment The present studies were supported by the Basic Science Research Institute Program, Ministry of Education, 1995, Project No. Copyright: Copyright 2018 Elsevier B.V., All rights reserved.

PY - 1997

Y1 - 1997

N2 - Binding characteristics of Bombyx mori nuclear polyhedrosis virus (BmNPV) to B. mori BmN-4 cells was determined. The cells had a single class of BmNPV-binding sites and displayed a low binding capacity for BmNPV in the range of 70 sites per cell. The biochemical nature of BmNPV-binding sites on the cell surface was identified. Treatment of BmN-4 cells with protease, wheat germ agglutinin, metabolic inhibitors, or lysosomotropic agents reduced BmNPV binding to BmN-4 cells, whereas treatment with phospholipase C showed no effect on virus binding. These results indicate that the structure of the binding site moiety is a protein-oligosaccharide complex.

AB - Binding characteristics of Bombyx mori nuclear polyhedrosis virus (BmNPV) to B. mori BmN-4 cells was determined. The cells had a single class of BmNPV-binding sites and displayed a low binding capacity for BmNPV in the range of 70 sites per cell. The biochemical nature of BmNPV-binding sites on the cell surface was identified. Treatment of BmN-4 cells with protease, wheat germ agglutinin, metabolic inhibitors, or lysosomotropic agents reduced BmNPV binding to BmN-4 cells, whereas treatment with phospholipase C showed no effect on virus binding. These results indicate that the structure of the binding site moiety is a protein-oligosaccharide complex.

KW - Binding site

KW - BmN-4 cells

KW - Bombyx mori nuclear polyhedrosis virus

UR - https://www.scopus.com/pages/publications/53249109545

U2 - 10.1007/BF02932465

DO - 10.1007/BF02932465

M3 - Article

AN - SCOPUS:53249109545

SN - 1226-8372

VL - 2

SP - 59

EP - 61

JO - Biotechnology and Bioprocess Engineering

JF - Biotechnology and Bioprocess Engineering

IS - 1

ER -

Biochemical analysis of BmNPV attachment to Bombyx Mori BmN-4 cells

Abstract

Bibliographical note

Keywords

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