Biotransformation of Pueraria lobata extract with Lactobacillus rhamnosus vitaP1 enhances anti-melanogenic activity

Jeong Eun Kwon; Jin Woo Lee; Yuna Park; Eun Hwa Sohn; Eui Su Choung; Seon A. Jang; Inhye Kim; Da Eun Lee; Hyun Jung Koo; Jong Phil Bak; Sung Ryul Lee; Se Chan Kang

doi:10.4014/jmb.1705.05087

Biotransformation of Pueraria lobata extract with Lactobacillus rhamnosus vitaP1 enhances anti-melanogenic activity

Jeong Eun Kwon, Jin Woo Lee, Yuna Park, Eun Hwa Sohn, Eui Su Choung, Seon A. Jang, Inhye Kim, Da Eun Lee, Hyun Jung Koo, Jong Phil Bak, Sung Ryul Lee, Se Chan Kang

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8 Citations (Scopus)

Abstract

Isoflavone itself is less available in the body without the aid of intestinal bacteria. In this study, we searched for isoflavone-transforming bacteria from human fecal specimens (n = 14) using differential selection media. Isoflavone-transforming activity as the production of dihydrogenistein and dihydrodaidzein was assessed by high-performance liquid chromatography and we found Lactobacillus rhamnosus, named L. rhamnosus vitaP1, through 16S rDNA sequence analysis. Extract from Pueraria lobata (EPL) and soy hypocotyl extract were fermented with L. rhamnosus vitaP1 for 24 and 48 h at 37oC. Fermented EPL (FEPL) showed enhanced anti-tyrosinase activity and antioxidant capacities, important suppressors of the pigmentation process, compared with that of EPL (p < 0.05). At up to 500 µg/ml of FEPL, there were no significant cell cytotoxicity and proliferation on B16-F10 melanoma cells. FEPL (100 µg/ml) could highly suppress the content of melanin and melanosome formation in B16- F10 cells. In summary, Lactobacillus rhamnosus vitaP1 was found to be able to biotransform isoflavones in EPL. FEPL showed augmented anti-melanogenic potential.

Original language	English
Pages (from-to)	22-31
Number of pages	10
Journal	Journal of Microbiology and Biotechnology
Volume	28
Issue number	1
DOIs	https://doi.org/10.4014/jmb.1705.05087
Publication status	Published - Jan 2018

Bibliographical note

Publisher Copyright:
© 2018 by The Korean Society for Microbiology and Biotechnology.

Keywords

Chemical analysis
Dihydrodaidzein
Dihydrogenistein
Lactobacillus rhamnosus
Microbiology
Pueraria lobata

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@article{212141638a6a4f00b3537d377cb54359,

title = "Biotransformation of Pueraria lobata extract with Lactobacillus rhamnosus vitaP1 enhances anti-melanogenic activity",

abstract = "Isoflavone itself is less available in the body without the aid of intestinal bacteria. In this study, we searched for isoflavone-transforming bacteria from human fecal specimens (n = 14) using differential selection media. Isoflavone-transforming activity as the production of dihydrogenistein and dihydrodaidzein was assessed by high-performance liquid chromatography and we found Lactobacillus rhamnosus, named L. rhamnosus vitaP1, through 16S rDNA sequence analysis. Extract from Pueraria lobata (EPL) and soy hypocotyl extract were fermented with L. rhamnosus vitaP1 for 24 and 48 h at 37oC. Fermented EPL (FEPL) showed enhanced anti-tyrosinase activity and antioxidant capacities, important suppressors of the pigmentation process, compared with that of EPL (p < 0.05). At up to 500 µg/ml of FEPL, there were no significant cell cytotoxicity and proliferation on B16-F10 melanoma cells. FEPL (100 µg/ml) could highly suppress the content of melanin and melanosome formation in B16- F10 cells. In summary, Lactobacillus rhamnosus vitaP1 was found to be able to biotransform isoflavones in EPL. FEPL showed augmented anti-melanogenic potential.",

keywords = "Chemical analysis, Dihydrodaidzein, Dihydrogenistein, Lactobacillus rhamnosus, Microbiology, Pueraria lobata",

author = "Kwon, {Jeong Eun} and Lee, {Jin Woo} and Yuna Park and Sohn, {Eun Hwa} and Choung, {Eui Su} and Jang, {Seon A.} and Inhye Kim and Lee, {Da Eun} and Koo, {Hyun Jung} and Bak, {Jong Phil} and Lee, {Sung Ryul} and Kang, {Se Chan}",

note = "Publisher Copyright: {\textcopyright} 2018 by The Korean Society for Microbiology and Biotechnology.",

year = "2018",

month = jan,

doi = "10.4014/jmb.1705.05087",

language = "English",

volume = "28",

pages = "22--31",

journal = "Journal of Microbiology and Biotechnology",

issn = "1017-7825",

publisher = "Korean Society for Microbiolog and Biotechnology",

number = "1",

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TY - JOUR

T1 - Biotransformation of Pueraria lobata extract with Lactobacillus rhamnosus vitaP1 enhances anti-melanogenic activity

AU - Kwon, Jeong Eun

AU - Lee, Jin Woo

AU - Park, Yuna

AU - Sohn, Eun Hwa

AU - Choung, Eui Su

AU - Jang, Seon A.

AU - Kim, Inhye

AU - Lee, Da Eun

AU - Koo, Hyun Jung

AU - Bak, Jong Phil

AU - Lee, Sung Ryul

AU - Kang, Se Chan

PY - 2018/1

Y1 - 2018/1

N2 - Isoflavone itself is less available in the body without the aid of intestinal bacteria. In this study, we searched for isoflavone-transforming bacteria from human fecal specimens (n = 14) using differential selection media. Isoflavone-transforming activity as the production of dihydrogenistein and dihydrodaidzein was assessed by high-performance liquid chromatography and we found Lactobacillus rhamnosus, named L. rhamnosus vitaP1, through 16S rDNA sequence analysis. Extract from Pueraria lobata (EPL) and soy hypocotyl extract were fermented with L. rhamnosus vitaP1 for 24 and 48 h at 37oC. Fermented EPL (FEPL) showed enhanced anti-tyrosinase activity and antioxidant capacities, important suppressors of the pigmentation process, compared with that of EPL (p < 0.05). At up to 500 µg/ml of FEPL, there were no significant cell cytotoxicity and proliferation on B16-F10 melanoma cells. FEPL (100 µg/ml) could highly suppress the content of melanin and melanosome formation in B16- F10 cells. In summary, Lactobacillus rhamnosus vitaP1 was found to be able to biotransform isoflavones in EPL. FEPL showed augmented anti-melanogenic potential.

AB - Isoflavone itself is less available in the body without the aid of intestinal bacteria. In this study, we searched for isoflavone-transforming bacteria from human fecal specimens (n = 14) using differential selection media. Isoflavone-transforming activity as the production of dihydrogenistein and dihydrodaidzein was assessed by high-performance liquid chromatography and we found Lactobacillus rhamnosus, named L. rhamnosus vitaP1, through 16S rDNA sequence analysis. Extract from Pueraria lobata (EPL) and soy hypocotyl extract were fermented with L. rhamnosus vitaP1 for 24 and 48 h at 37oC. Fermented EPL (FEPL) showed enhanced anti-tyrosinase activity and antioxidant capacities, important suppressors of the pigmentation process, compared with that of EPL (p < 0.05). At up to 500 µg/ml of FEPL, there were no significant cell cytotoxicity and proliferation on B16-F10 melanoma cells. FEPL (100 µg/ml) could highly suppress the content of melanin and melanosome formation in B16- F10 cells. In summary, Lactobacillus rhamnosus vitaP1 was found to be able to biotransform isoflavones in EPL. FEPL showed augmented anti-melanogenic potential.

KW - Chemical analysis

KW - Dihydrodaidzein

KW - Dihydrogenistein

KW - Lactobacillus rhamnosus

KW - Microbiology

KW - Pueraria lobata

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U2 - 10.4014/jmb.1705.05087

DO - 10.4014/jmb.1705.05087

M3 - Article

C2 - 29081087

AN - SCOPUS:85043578414

SN - 1017-7825

VL - 28

SP - 22

EP - 31

JO - Journal of Microbiology and Biotechnology

JF - Journal of Microbiology and Biotechnology

IS - 1

ER -

Biotransformation of Pueraria lobata extract with Lactobacillus rhamnosus vitaP1 enhances anti-melanogenic activity

Abstract

Bibliographical note

Keywords

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