Bone morphogenic protein-2 (BMP-2) immobilized biodegradable scaffolds for bone tissue engineering

Eun Kim Sung; Kyun Rha Hyung; Sibin Surendran; Whan Han Chang; Cheon Lee Sang; Woo Choi Hyung; Yong Woo Choi; Kweon Haeng Lee; Won Rhie Jong; Tae Ahn Sang

doi:10.1007/bf03218725

Bone morphogenic protein-2 (BMP-2) immobilized biodegradable scaffolds for bone tissue engineering

Eun Kim Sung, Kyun Rha Hyung, Sibin Surendran, Whan Han Chang, Cheon Lee Sang, Woo Choi Hyung, Yong Woo Choi, Kweon Haeng Lee, Won Rhie Jong, Tae Ahn Sang

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

Recombinant human bone morphogenic protein-2 (rhBMP-2), which is known as one of the major local stimuli for osteogenic differentiation, was immobilized on the surface of hyaluronic acid (HA)-modified poly(ε-caprolactone) (PCL) (HA-PCL) scaffolds to improve the attachment, proliferation, and differentiation of human bone marrow stem cells (hBMSCs) for bone tissue engineering. The rhBMP-2 proteins were directly immobilized onto the HA-modified PCL scaffolds by the chemical grafting the amine groups of proteins to carboxylic acid groups of HA. The amount of covalently bounded rhBMP-2 was measured to 1.6 pg/mg (rhBMP/HA-PCL scaffold) by using a sandwich enzyme-linked immunosorbant assay. The rhBMP-2 immobilized HA-modified-PCL scaffold exhibited the good colonization, by the newly differentiated osteoblasts, with a statistically significant increase of the rhBMP-2 release and alkaline phosphatase activity as compared with the control groups both PCL and HA-PCL scaffolds. We also found enhanced mineralization and elevated osteocalcin detection for the rhBMP-2 immobilized HA-PCL scaffolds, in vitro.

Original language	English
Pages (from-to)	565-572
Number of pages	8
Journal	Macromolecular Research
Volume	14
Issue number	5
DOIs	https://doi.org/10.1007/bf03218725
Publication status	Published - Oct 2006

Keywords

Bone tissue engineering
Human mesenchymal stem cells
Recombinant human bone morphogenetic protein-2
Scaffolds
Surface modification

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title = "Bone morphogenic protein-2 (BMP-2) immobilized biodegradable scaffolds for bone tissue engineering",

abstract = "Recombinant human bone morphogenic protein-2 (rhBMP-2), which is known as one of the major local stimuli for osteogenic differentiation, was immobilized on the surface of hyaluronic acid (HA)-modified poly(ε-caprolactone) (PCL) (HA-PCL) scaffolds to improve the attachment, proliferation, and differentiation of human bone marrow stem cells (hBMSCs) for bone tissue engineering. The rhBMP-2 proteins were directly immobilized onto the HA-modified PCL scaffolds by the chemical grafting the amine groups of proteins to carboxylic acid groups of HA. The amount of covalently bounded rhBMP-2 was measured to 1.6 pg/mg (rhBMP/HA-PCL scaffold) by using a sandwich enzyme-linked immunosorbant assay. The rhBMP-2 immobilized HA-modified-PCL scaffold exhibited the good colonization, by the newly differentiated osteoblasts, with a statistically significant increase of the rhBMP-2 release and alkaline phosphatase activity as compared with the control groups both PCL and HA-PCL scaffolds. We also found enhanced mineralization and elevated osteocalcin detection for the rhBMP-2 immobilized HA-PCL scaffolds, in vitro.",

keywords = "Bone tissue engineering, Human mesenchymal stem cells, Recombinant human bone morphogenetic protein-2, Scaffolds, Surface modification",

author = "Sung, {Eun Kim} and Hyung, {Kyun Rha} and Sibin Surendran and Chang, {Whan Han} and Sang, {Cheon Lee} and Hyung, {Woo Choi} and Choi, {Yong Woo} and Lee, {Kweon Haeng} and Jong, {Won Rhie} and Sang, {Tae Ahn}",

year = "2006",

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doi = "10.1007/bf03218725",

language = "English",

volume = "14",

pages = "565--572",

journal = "Macromolecular Research",

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AU - Sung, Eun Kim

AU - Hyung, Kyun Rha

AU - Surendran, Sibin

AU - Chang, Whan Han

AU - Sang, Cheon Lee

AU - Hyung, Woo Choi

AU - Choi, Yong Woo

AU - Lee, Kweon Haeng

AU - Jong, Won Rhie

AU - Sang, Tae Ahn

PY - 2006/10

Y1 - 2006/10

N2 - Recombinant human bone morphogenic protein-2 (rhBMP-2), which is known as one of the major local stimuli for osteogenic differentiation, was immobilized on the surface of hyaluronic acid (HA)-modified poly(ε-caprolactone) (PCL) (HA-PCL) scaffolds to improve the attachment, proliferation, and differentiation of human bone marrow stem cells (hBMSCs) for bone tissue engineering. The rhBMP-2 proteins were directly immobilized onto the HA-modified PCL scaffolds by the chemical grafting the amine groups of proteins to carboxylic acid groups of HA. The amount of covalently bounded rhBMP-2 was measured to 1.6 pg/mg (rhBMP/HA-PCL scaffold) by using a sandwich enzyme-linked immunosorbant assay. The rhBMP-2 immobilized HA-modified-PCL scaffold exhibited the good colonization, by the newly differentiated osteoblasts, with a statistically significant increase of the rhBMP-2 release and alkaline phosphatase activity as compared with the control groups both PCL and HA-PCL scaffolds. We also found enhanced mineralization and elevated osteocalcin detection for the rhBMP-2 immobilized HA-PCL scaffolds, in vitro.

AB - Recombinant human bone morphogenic protein-2 (rhBMP-2), which is known as one of the major local stimuli for osteogenic differentiation, was immobilized on the surface of hyaluronic acid (HA)-modified poly(ε-caprolactone) (PCL) (HA-PCL) scaffolds to improve the attachment, proliferation, and differentiation of human bone marrow stem cells (hBMSCs) for bone tissue engineering. The rhBMP-2 proteins were directly immobilized onto the HA-modified PCL scaffolds by the chemical grafting the amine groups of proteins to carboxylic acid groups of HA. The amount of covalently bounded rhBMP-2 was measured to 1.6 pg/mg (rhBMP/HA-PCL scaffold) by using a sandwich enzyme-linked immunosorbant assay. The rhBMP-2 immobilized HA-modified-PCL scaffold exhibited the good colonization, by the newly differentiated osteoblasts, with a statistically significant increase of the rhBMP-2 release and alkaline phosphatase activity as compared with the control groups both PCL and HA-PCL scaffolds. We also found enhanced mineralization and elevated osteocalcin detection for the rhBMP-2 immobilized HA-PCL scaffolds, in vitro.

KW - Bone tissue engineering

KW - Human mesenchymal stem cells

KW - Recombinant human bone morphogenetic protein-2

KW - Scaffolds

KW - Surface modification

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Bone morphogenic protein-2 (BMP-2) immobilized biodegradable scaffolds for bone tissue engineering

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Keywords

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