Brevundimonas basaltis sp. nov., isolated from black sand

A Gram-negative, aerobic, rod-shaped, motile Brevundimonas-like bacterial strain, J22^T, was isolated from black sand collected from Soesoggak, Jeju Island, Korea. Growth of strain J22^T was observed in R2A medium at temperatures between 10 and 42°C (optimum 30°C), between pH 6.5 and 10.5 (optimum pH 7.5) and at a NaCl concentration between 0 and 4% (w/v) (optimum 0-1 %). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain J22^T belonged to the genus Brevundimonas, with high sequence similarities of >97% to the sequence of the type strains Brevundimonas alba CB88^T, Brevundimonas lenta DS-18^T, Brevundimonas variabilis CB17^T, Mycoplana bullata TK0051^T, Brevundimonas kwangchunensis KSL-102^T, Brevundimonas intermedia CB63^T, Brevundimonas subvibrioides CB81^T and Brevundimonas bacteroides CB7^T. Strain J22^T exhibited DNA-DNA relatedness values of less than 22.2% with the phylogenetically related species of the genus Brevundimonas. The DNA G+C content of strain J22^T was 66.3 mol%. The predominant cellular fatty acids were C_18:1ω7c, C_16:0 and C_16:1ω9c; C_12:0 3-OH was present, which chemotaxonomically characterizes the members of the genus Brevundimonas. Phylogenetic, genomic and biochemical characteristics served to differentiate this isolate from recognized members of the genus Brevundimonas. Strain J22^T (=KCTC 22177^T=JCM 15911^T) should be classified as a novel species in the genus Brevundimonas, for which the name Brevundimonas basaltis sp. nov. is proposed.