Calcium Carbonate Mineralized Nanoparticles as an Intracellular Transporter of Cytochrome c for Cancer Therapy

Ahn Na Koo; Kyung Hyun Min; Hong Jae Lee; Jun Ho Jegal; Jae Won Lee; Sang Cheon Lee

doi:10.1002/asia.201500630

Calcium Carbonate Mineralized Nanoparticles as an Intracellular Transporter of Cytochrome c for Cancer Therapy

Ahn Na Koo, Kyung Hyun Min, Hong Jae Lee, Jun Ho Jegal, Jae Won Lee, Sang Cheon Lee

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

A new intracellular delivery system based on an apoptotic protein-loaded calcium carbonate (CaCO₃) mineralized nanoparticle (MNP) is described. Apoptosis-inducing cytochrome c (Cyt c) loaded CaCO₃ MNPs (Cyt c MNPs) were prepared by block copolymer mediated in situ CaCO₃ mineralization in the presence of Cyt c. The resulting Cyt c MNPs had a vaterite polymorph of CaCO₃ with a mean hydrodynamic diameter of 360.5 nm and exhibited 60?% efficiency for Cyt c loading. The Cyt c MNPs were stable at physiological pH (pH 7.4) and effectively prohibited the release of Cyt c, whereas, at intracellular endosomal pH (pH 5.0), Cyt c release was facilitated. The MNPs enable the endosomal escape of Cyt c for effective localization of Cyt c in the cytosols of MCF-7 cells. Flow cytometry showed that the Cyt c MNPs effectively induced apoptosis of MCF-7 cells. These findings indicate that the CaCO₃ MNPs can meet the prerequisites for delivery of cell-impermeable therapeutic proteins for cancer therapy.

Original language	English
Pages (from-to)	2380-2387
Number of pages	8
Journal	Chemistry - An Asian Journal
Volume	10
Issue number	11
DOIs	https://doi.org/10.1002/asia.201500630
Publication status	Published - 1 Nov 2015

Bibliographical note

Publisher Copyright:
© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Keywords

apoptosis
calcium
drug delivery
nanoparticles
proteins

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1002/asia.201500630

Cite this

@article{08bc83bb201540b68f2f8dc78903793e,

title = "Calcium Carbonate Mineralized Nanoparticles as an Intracellular Transporter of Cytochrome c for Cancer Therapy",

abstract = "A new intracellular delivery system based on an apoptotic protein-loaded calcium carbonate (CaCO3) mineralized nanoparticle (MNP) is described. Apoptosis-inducing cytochrome c (Cyt c) loaded CaCO3 MNPs (Cyt c MNPs) were prepared by block copolymer mediated in situ CaCO3 mineralization in the presence of Cyt c. The resulting Cyt c MNPs had a vaterite polymorph of CaCO3 with a mean hydrodynamic diameter of 360.5 nm and exhibited 60?% efficiency for Cyt c loading. The Cyt c MNPs were stable at physiological pH (pH 7.4) and effectively prohibited the release of Cyt c, whereas, at intracellular endosomal pH (pH 5.0), Cyt c release was facilitated. The MNPs enable the endosomal escape of Cyt c for effective localization of Cyt c in the cytosols of MCF-7 cells. Flow cytometry showed that the Cyt c MNPs effectively induced apoptosis of MCF-7 cells. These findings indicate that the CaCO3 MNPs can meet the prerequisites for delivery of cell-impermeable therapeutic proteins for cancer therapy.",

keywords = "apoptosis, calcium, drug delivery, nanoparticles, proteins",

author = "Koo, {Ahn Na} and Min, {Kyung Hyun} and Lee, {Hong Jae} and Jegal, {Jun Ho} and Lee, {Jae Won} and Lee, {Sang Cheon}",

note = "Publisher Copyright: {\textcopyright} 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.",

year = "2015",

month = nov,

day = "1",

doi = "10.1002/asia.201500630",

language = "English",

volume = "10",

pages = "2380--2387",

journal = "Chemistry - An Asian Journal",

issn = "1861-4728",

publisher = "John Wiley and Sons Ltd",

number = "11",

}

TY - JOUR

T1 - Calcium Carbonate Mineralized Nanoparticles as an Intracellular Transporter of Cytochrome c for Cancer Therapy

AU - Koo, Ahn Na

AU - Min, Kyung Hyun

AU - Lee, Hong Jae

AU - Jegal, Jun Ho

AU - Lee, Jae Won

AU - Lee, Sang Cheon

PY - 2015/11/1

Y1 - 2015/11/1

N2 - A new intracellular delivery system based on an apoptotic protein-loaded calcium carbonate (CaCO3) mineralized nanoparticle (MNP) is described. Apoptosis-inducing cytochrome c (Cyt c) loaded CaCO3 MNPs (Cyt c MNPs) were prepared by block copolymer mediated in situ CaCO3 mineralization in the presence of Cyt c. The resulting Cyt c MNPs had a vaterite polymorph of CaCO3 with a mean hydrodynamic diameter of 360.5 nm and exhibited 60?% efficiency for Cyt c loading. The Cyt c MNPs were stable at physiological pH (pH 7.4) and effectively prohibited the release of Cyt c, whereas, at intracellular endosomal pH (pH 5.0), Cyt c release was facilitated. The MNPs enable the endosomal escape of Cyt c for effective localization of Cyt c in the cytosols of MCF-7 cells. Flow cytometry showed that the Cyt c MNPs effectively induced apoptosis of MCF-7 cells. These findings indicate that the CaCO3 MNPs can meet the prerequisites for delivery of cell-impermeable therapeutic proteins for cancer therapy.

AB - A new intracellular delivery system based on an apoptotic protein-loaded calcium carbonate (CaCO3) mineralized nanoparticle (MNP) is described. Apoptosis-inducing cytochrome c (Cyt c) loaded CaCO3 MNPs (Cyt c MNPs) were prepared by block copolymer mediated in situ CaCO3 mineralization in the presence of Cyt c. The resulting Cyt c MNPs had a vaterite polymorph of CaCO3 with a mean hydrodynamic diameter of 360.5 nm and exhibited 60?% efficiency for Cyt c loading. The Cyt c MNPs were stable at physiological pH (pH 7.4) and effectively prohibited the release of Cyt c, whereas, at intracellular endosomal pH (pH 5.0), Cyt c release was facilitated. The MNPs enable the endosomal escape of Cyt c for effective localization of Cyt c in the cytosols of MCF-7 cells. Flow cytometry showed that the Cyt c MNPs effectively induced apoptosis of MCF-7 cells. These findings indicate that the CaCO3 MNPs can meet the prerequisites for delivery of cell-impermeable therapeutic proteins for cancer therapy.

KW - apoptosis

KW - calcium

KW - drug delivery

KW - nanoparticles

KW - proteins

UR - http://www.scopus.com/inward/record.url?scp=84945484190&partnerID=8YFLogxK

U2 - 10.1002/asia.201500630

DO - 10.1002/asia.201500630

M3 - Article

C2 - 26235642

AN - SCOPUS:84945484190

SN - 1861-4728

VL - 10

SP - 2380

EP - 2387

JO - Chemistry - An Asian Journal

JF - Chemistry - An Asian Journal

IS - 11

ER -

Calcium Carbonate Mineralized Nanoparticles as an Intracellular Transporter of Cytochrome c for Cancer Therapy

Abstract

Bibliographical note

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this