TY - JOUR
T1 - Callus induction and in vitro mass culture of adventitious roots from leaf segment explants of Dendropanax morbifera Lev.
AU - Sukweenadhi, J.
AU - Choi, J. Y.
AU - Kim, Y. J.
AU - Kaliraj, L.
AU - Abid, S.
AU - Ahn, J. C.
AU - Yang, D. C.
N1 - Funding Information:
This research was supported by Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (KIPET Grant No. 31700-3), Republic of Korea. This article does not contain any studies with human participants or animal performed by the authors.
Publisher Copyright:
© 2019 Published under licence by IOP Publishing Ltd.
PY - 2019/6/27
Y1 - 2019/6/27
N2 - Dendropanax morbifera Lev. is a unique species and natively found in Korea and distributed in the South regions, such as Jeju, Goheung, and Wando. In this study, tissue culture system for native D. morbifera was developed. The callus from native D. morbifera leaves was cultured on Woody Plant Media (WPM) supplemented with 30 g L-1 of sucrose, with addition of 2.4-D and bA [(0.5, 1.0, 2.0) mg L-1], separately and mixed. After 5 wk of culture, the highest induction of callus was obtained from 0.5 mg L-1 of 2.4-D mixed with 2.0 mg L1 of BA. Adventitious root formation on different media (MS, WPM, B5) with various auxins (IBA, IAA, and NAA) and different concentration [(0, 1, 3, 5) mg L-1] were tested. After 8 wk of culture, WPM showed better induction of adventitious root. The highest induction of adventitious root was obtained on 3.0 mg L-1 IBA. Root growth was best in WPM liquid medium with 3.0 mg L-1 of IBA and 30 g L-1 of sucrose. The same formulation with modified 1/2 WPM was successfully established in vitro adventitious roots culture in 18-L bioreactor system. This study also proposed as the mass production technique of adventitious roots from native D. morbifera.
AB - Dendropanax morbifera Lev. is a unique species and natively found in Korea and distributed in the South regions, such as Jeju, Goheung, and Wando. In this study, tissue culture system for native D. morbifera was developed. The callus from native D. morbifera leaves was cultured on Woody Plant Media (WPM) supplemented with 30 g L-1 of sucrose, with addition of 2.4-D and bA [(0.5, 1.0, 2.0) mg L-1], separately and mixed. After 5 wk of culture, the highest induction of callus was obtained from 0.5 mg L-1 of 2.4-D mixed with 2.0 mg L1 of BA. Adventitious root formation on different media (MS, WPM, B5) with various auxins (IBA, IAA, and NAA) and different concentration [(0, 1, 3, 5) mg L-1] were tested. After 8 wk of culture, WPM showed better induction of adventitious root. The highest induction of adventitious root was obtained on 3.0 mg L-1 IBA. Root growth was best in WPM liquid medium with 3.0 mg L-1 of IBA and 30 g L-1 of sucrose. The same formulation with modified 1/2 WPM was successfully established in vitro adventitious roots culture in 18-L bioreactor system. This study also proposed as the mass production technique of adventitious roots from native D. morbifera.
KW - Adventitious roots
KW - bioreactor system
KW - gingseng tree
KW - in vitro culture
KW - tissue culture
UR - http://www.scopus.com/inward/record.url?scp=85069050386&partnerID=8YFLogxK
U2 - 10.1088/1755-1315/293/1/012024
DO - 10.1088/1755-1315/293/1/012024
M3 - Conference article
AN - SCOPUS:85069050386
VL - 293
JO - IOP Conference Series: Earth and Environmental Science
JF - IOP Conference Series: Earth and Environmental Science
SN - 1755-1307
IS - 1
M1 - 012024
T2 - 2nd International Conference on Natural Resources and Life Sciences, NRLS 2018
Y2 - 23 August 2018 through 24 August 2018
ER -