Abstract
We developed a centrifugal microfluidic system to detect four kinds of food poisoning bacteria. A loopmediated isothermal amplification (LAMP) method and a simplified POC microdevice were adopted as a rapid and portable genetic analysis. Eriochrome Black T (EBT) which is a metal indicator for Mg2+ was used as a colorimetric detection reagent to confirm the produced amplicons with the naked eyes. The proposed microdevice platform provides an advanced multiplex and rapid point-of-care diagnostic tool for early food poisoning bacteria analysis.
| Original language | English |
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| Title of host publication | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 1006-1008 |
| Number of pages | 3 |
| ISBN (Electronic) | 9780979806476 |
| Publication status | Published - 2014 |
| Event | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States Duration: 26 Oct 2014 → 30 Oct 2014 |
Publication series
| Name | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 |
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Conference
| Conference | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 |
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| Country/Territory | United States |
| City | San Antonio |
| Period | 26/10/14 → 30/10/14 |
Bibliographical note
Publisher Copyright:© 14CBMS.
Keywords
- Centrifugal microfluidic system
- Colorimetric detection
- Food poisoning bacteria
- Loop-mediated isothermal amplification
- Point-of-care microdevice