Abstract
We developed a centrifugal microdevice for real-time identification of three food poisoning bacteria from real food samples. The use of the recombinase polymerase amplification (RPA) method and direct PCR buffer eliminates the sample pretreatment step. The entire analysis processes including aliquoting of samples and reagents, target amplification, and real time detection were conducted with a portable rotary genetic analyzer within 30 minutes. The proposed centrifugal microdevice platform provides a rapid and miniaturized point-of-care diagnostic tool for on-site food poisoning bacteria detection.
| Original language | English |
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| Title of host publication | MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 1187-1189 |
| Number of pages | 3 |
| ISBN (Electronic) | 9780979806483 |
| Publication status | Published - 2015 |
| Event | 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 - Gyeongju, Korea, Republic of Duration: 25 Oct 2015 → 29 Oct 2015 |
Publication series
| Name | MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
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Conference
| Conference | 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 |
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| Country/Territory | Korea, Republic of |
| City | Gyeongju |
| Period | 25/10/15 → 29/10/15 |
Bibliographical note
Publisher Copyright:© 15CBMS-0001.
Keywords
- Centrifugal microfluidic system
- Direct isothermal amplification
- Food poisoning bacteria
- Point-of-care microdevice
- Recombinase polymerase amplification