Ceramide is involved in MPP+-induced cytotoxicity in human neuroblastoma cells

Eun Joo Nam, Hye Sook Lee, Young Jae Lee, Wan Seok Joo, Sungho Maeng, Hye In Im, Chan Woong Park, Yong Sik Kim

Research output: Contribution to journalReview articlepeer-review

3 Citations (Scopus)

Abstract

To understand the cytotoxic mechanism of MPP+, we examined the involvement of ceramide in MPP+-induced cytotoxicity to human neuroblastoma SH-SY5Y cells. When MPP+ was exposed to SH-SY5Y cells, MPP+ induced dose-dependant cytotoxicity accompanied by an elevation of intracellular ceramide levels by 2-fold in SH-SY5Y cells. Three methods were used to test the hypothesis that the elevated intracellular ceramide is related to MPP+-induced cytotoxicity: C2-ceramide was directly applied to cells, sphingomyelinase (SMase) was exogenously added, and oleoylethanolamine (OE) was used to inhibit degradation of ceramide. Furthermore, inhibition of ceramide-activated protein phosphatase (CAPP), the effector of ceramide, using okadaic acid (OA) attenuated cell death but treatment of fumonisin B1, the ceramide synthase inhibitor, did not alter the cytotoxic effect of MPP+. Based on these, we suggest that the elevation of intracellular ceramide is one of the important mediators in MPP+-induced cell death.

Original languageEnglish
Pages (from-to)281-286
Number of pages6
JournalKorean Journal of Physiology and Pharmacology
Volume6
Issue number6
Publication statusPublished - Dec 2002

Keywords

  • Ceramide-activated protein phosphate
  • Dopaminergic neuroblastoma
  • MPP
  • Sphingomyelinase

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