Changes in chemical characteristics of cellulase-treated wheat germ extract

Jae Kang Lee, Davin Jang, Dongwoo Kang, Jeonghoon Lee, Hyeim Kum, Yonghyoun Choi, Hee Kang, Yong Seok Choi, Dae Ok Kim

Research output: Contribution to journalArticlepeer-review


Wheat germ, which is rich in nutrients and phytochemicals, is a by-product during the milling process of wheat kernel. In this study, we aimed to increase the amount of bioactive 2,6-dimethoxy-1,4-benzoquinone (2,6-DMBQ) in wheat germ using the cell-wall-degrading enzyme cellulase (Celluclast 1.5L). The amounts of organic acids, free sugars, and 2,6-DMBQ in wheat germ treated with Celluclast 1.5L were evaluated at various reaction times and temperatures. The results of reversed-phase high-performance liquid chromatography of Celluclast 1.5L-treated wheat germ revealed 2,6-DMBQ, four organic acids (tartaric, acetic, lactic, and succinic acids), and three free sugars (sucrose, fructose, and glucose). As reaction time and temperature of the mixture of wheat germ and Celluclast 1.5L increased, the contents of four organic acids, glucose, fructose, and 2,6-DMBQ increased, but that of sucrose decreased. Taken together, these results suggest that Celluclast 1.5L-treated wheat germ containing increased amounts of 2,6-DMBQ serves as a source of functional ingredients in food industry.

Original languageEnglish
Pages (from-to)97-102
Number of pages6
JournalKorean Journal of Food Science and Technology
Issue number2
Publication statusPublished - 2019


  • By-product, 2,6-dimethoxy-1,4-benzoquinone
  • High-performance liquid chromatography
  • Organic acids
  • Wheat germ


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