Abstract
In this study, PVF11 was selected among 20 candidate pathogenesis-related genes in Burkholderia glumae based on its effect on virulence to rice. PVF11 was found to interact with several plant defense-related WRKY proteins as evidenced through yeast-two hybrid analysis (Y2H). Moreover, PVF11 showed interactions with abiotic and biotic stress response-related rice proteins, as shown by genome-wide Y2H screening employing PVF11 and a cDNA library from B. glumae-infected rice. To confirm the effect of PVF11 on B. glumae virulence, in planta assays were conducted at different stages of rice growth. As a result, a PVF11-defective mutant showed reduced virulence in rice seedlings and stems but not in rice panicles, indicating that PVF11 involvement in B. glumae virulence in rice is stage-dependent.
| Original language | English |
|---|---|
| Pages (from-to) | 280-286 |
| Number of pages | 7 |
| Journal | Plant Pathology Journal |
| Volume | 35 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2019 |
Bibliographical note
Publisher Copyright:© The Korean Society of Plant Pathology.
Keywords
- Burkholderia glumae
- Virulence
- Yeast two hybrid analysis
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