Cloning and characterization of GITR ligand

J. D. Kim; B. K. Choi; J. S. Bae; U. H. Lee; I. S. Han; H. W. Lee; B. S. Youn; D. S. Vinay; B. S. Kwon

doi:10.1038/sj.gene.6364026

Cloning and characterization of GITR ligand

J. D. Kim
, B. K. Choi
, J. S. Bae
, U. H. Lee
, I. S. Han
, H. W. Lee
, B. S. Youn
, D. S. Vinay
, B. S. Kwon

Research output: Contribution to journal › Article › peer-review

102 Citations (Scopus)

Abstract

The gene encoding the natural ligand of murine glucocorticoid-induced tumor necrosis factor receptor (GITR) was cloned and characterized. The putative GITR ligand (GITRL) is composed of 173 amino acids with features resembling those of type II membrane proteins and is 51% identical to the human activation-inducible TNF receptor (AITR) ligand, TL6. Expression of the GITRL is restricted to immature and mature splenic dendritic cells. GITRL binds GITR expressed on HEK 293 cells and triggers NF-κB activation. Functional studies reveal that soluble CD8-GITRL prevents CD4+ CD25+ regulatory T-cell-mediated suppressive activities.

Original language	English
Pages (from-to)	564-569
Number of pages	6
Journal	Genes and Immunity
Volume	4
Issue number	8
DOIs	https://doi.org/10.1038/sj.gene.6364026
Publication status	Published - Dec 2003

Bibliographical note

Funding Information:
We thank Dr Shimon Sakaguchi for providing the DTA-1 hybridoma. This work was supported by the SRC Fund to the IRC at the University of Ulsan from KOSEF and the Ministry of Science and Technology, Korea, and US Public Health Service Grants R01EY013325 and P30EY02377 from the National Eye Institute, National Institutes of Health, Bethesda, MD, USA

Keywords

Cell surface molecules
Dendritic cells
Rodent
Suppression

Access to Document

10.1038/sj.gene.6364026

Cite this

@article{16341557e4dc4c0099e104be7080602b,

title = "Cloning and characterization of GITR ligand",

abstract = "The gene encoding the natural ligand of murine glucocorticoid-induced tumor necrosis factor receptor (GITR) was cloned and characterized. The putative GITR ligand (GITRL) is composed of 173 amino acids with features resembling those of type II membrane proteins and is 51\% identical to the human activation-inducible TNF receptor (AITR) ligand, TL6. Expression of the GITRL is restricted to immature and mature splenic dendritic cells. GITRL binds GITR expressed on HEK 293 cells and triggers NF-κB activation. Functional studies reveal that soluble CD8-GITRL prevents CD4+ CD25+ regulatory T-cell-mediated suppressive activities.",

keywords = "Cell surface molecules, Dendritic cells, Rodent, Suppression",

author = "Kim, \{J. D.\} and Choi, \{B. K.\} and Bae, \{J. S.\} and Lee, \{U. H.\} and Han, \{I. S.\} and Lee, \{H. W.\} and Youn, \{B. S.\} and Vinay, \{D. S.\} and Kwon, \{B. S.\}",

note = "Funding Information: We thank Dr Shimon Sakaguchi for providing the DTA-1 hybridoma. This work was supported by the SRC Fund to the IRC at the University of Ulsan from KOSEF and the Ministry of Science and Technology, Korea, and US Public Health Service Grants R01EY013325 and P30EY02377 from the National Eye Institute, National Institutes of Health, Bethesda, MD, USA",

year = "2003",

month = dec,

doi = "10.1038/sj.gene.6364026",

language = "English",

volume = "4",

pages = "564--569",

journal = "Genes and Immunity",

issn = "1466-4879",

publisher = "Springer Nature",

number = "8",

}

TY - JOUR

T1 - Cloning and characterization of GITR ligand

AU - Kim, J. D.

AU - Choi, B. K.

AU - Bae, J. S.

AU - Lee, U. H.

AU - Han, I. S.

AU - Lee, H. W.

AU - Youn, B. S.

AU - Vinay, D. S.

AU - Kwon, B. S.

N1 - Funding Information: We thank Dr Shimon Sakaguchi for providing the DTA-1 hybridoma. This work was supported by the SRC Fund to the IRC at the University of Ulsan from KOSEF and the Ministry of Science and Technology, Korea, and US Public Health Service Grants R01EY013325 and P30EY02377 from the National Eye Institute, National Institutes of Health, Bethesda, MD, USA

PY - 2003/12

Y1 - 2003/12

N2 - The gene encoding the natural ligand of murine glucocorticoid-induced tumor necrosis factor receptor (GITR) was cloned and characterized. The putative GITR ligand (GITRL) is composed of 173 amino acids with features resembling those of type II membrane proteins and is 51% identical to the human activation-inducible TNF receptor (AITR) ligand, TL6. Expression of the GITRL is restricted to immature and mature splenic dendritic cells. GITRL binds GITR expressed on HEK 293 cells and triggers NF-κB activation. Functional studies reveal that soluble CD8-GITRL prevents CD4+ CD25+ regulatory T-cell-mediated suppressive activities.

AB - The gene encoding the natural ligand of murine glucocorticoid-induced tumor necrosis factor receptor (GITR) was cloned and characterized. The putative GITR ligand (GITRL) is composed of 173 amino acids with features resembling those of type II membrane proteins and is 51% identical to the human activation-inducible TNF receptor (AITR) ligand, TL6. Expression of the GITRL is restricted to immature and mature splenic dendritic cells. GITRL binds GITR expressed on HEK 293 cells and triggers NF-κB activation. Functional studies reveal that soluble CD8-GITRL prevents CD4+ CD25+ regulatory T-cell-mediated suppressive activities.

KW - Cell surface molecules

KW - Dendritic cells

KW - Rodent

KW - Suppression

UR - https://www.scopus.com/pages/publications/1642338649

U2 - 10.1038/sj.gene.6364026

DO - 10.1038/sj.gene.6364026

M3 - Article

C2 - 14647196

AN - SCOPUS:1642338649

SN - 1466-4879

VL - 4

SP - 564

EP - 569

JO - Genes and Immunity

JF - Genes and Immunity

IS - 8

ER -

Cloning and characterization of GITR ligand

Abstract

Bibliographical note

Keywords

Access to Document

Other files and links

Fingerprint

Cite this