Abstract
Objective: The purpose of this study was to compare the oral microbiome of siblings with and without dental caries using next-generation sequencing. Materials and Methods: To investigate the oral microbiome composition, 14 young siblings, seven with caries and seven without, were enrolled from seven sibling-pair families. Supragingival plaque samples were collected from the cervicobuccal area of posterior teeth. All samples were analyzed by pyrosequencing, based on the 16S rRNA gene hypervariable regions, V1–V4. Results: The organisms identified belonged to 65 genera. Fifty-two genera were identified in the subjects with caries and 58 in those without; 45 genera were shared by both groups. In the Shannon index, the caries group showed lower bacterial diversity than the caries-free group and the difference was significant (Wilcoxon signed-rank test, P < 0.05). Additionally, similarities between siblings were evident in analyses based on weighted UniFrac distances (P < 0.05). Conclusions: In this study, the diversity of the microbiome was reduced in subjects with dental caries, while similarity between siblings seemed to be retained.
Original language | English |
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Pages (from-to) | 549-556 |
Number of pages | 8 |
Journal | Oral Diseases |
Volume | 22 |
Issue number | 6 |
DOIs | |
Publication status | Published - 2016 |
Bibliographical note
Publisher Copyright:© 2016 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd
Keywords
- dental caries
- next-generation sequencing
- oral microbiome
- pyrosequencing
- sibling