Abstract
The performance of CNT network junction probe and conventional dialysis electrode was compared for the efficiency of real time glutamate monitoring in 11 vessel occlusion (11 VO) rat model. The dialysis electrode (Sycopel Scientific Ltd., NE 38 9BZ, U.K.) was perfused with fresh phosphate buffer saline (PBS) solution containing glutamate oxidase at 0.5 μL/min during the measurement. The glutamate oxidase immobilized CNT probe was implanted in the brain of the rat model. From the results of real time glutamate monitoring and cerebral blood flow (CBF) in 11 VO rat model, it seemed that the dynamics of glutamate release in extracellular fluid were similar both by dialysis electrode or CNT probe. However, the response of CNT probe based biosensor with L-glutamate oxi-dase (GLOD) to glutamate was faster (<10 sec) than that of dialysis electrode (>70 sec). The sensitivity of CNT probe to glutamate was higher than that of dialysis electrode in the rat model (approx. 20 times). Therefore, we suggest that the GLOD-immobilized CNT probe provides a fast response time and a high sensitivity for glutamate which are essential for the real-time glutamate monitoring.
| Original language | English |
|---|---|
| Pages (from-to) | 82-86 |
| Number of pages | 5 |
| Journal | Biochip Journal |
| Volume | 3 |
| Issue number | 1 |
| Publication status | Published - Mar 2009 |
Keywords
- CNT probe
- Dialysis electrode
- Neurotransmitter
- Real-time monitoring