Concentrating rotavirus and recombinant enhanced green fluorescent protein from E. coli using a temperature-sensitive hydrogel

Kynng Hwa Chang; Jong Hwa Park; Chang Ho Park; In Sik Chung

doi:10.1007/BF02931934

Concentrating rotavirus and recombinant enhanced green fluorescent protein from E. coli using a temperature-sensitive hydrogel

Kynng Hwa Chang
, Jong Hwa Park
, Chang Ho Park
, In Sik Chung

Research output: Contribution to journal › Article › peer-review

Abstract

Recombinant enhanced green fluorescent protein (EGFP) from E. coli was concentrated 1.9 times by ultrafiltration using a temperature-sensitive hydrogel. Protein recovery and separation efficiency were 64% and 45%, respectively. Increased concentration of recombinant EGFP was confirmed by SDS-PAGE. Rotavirus was concentrated 3.2 times by ultrafiltration using a temperature-sensitive hydrogel, at 95% of virus recovery and 93% of separation efficiency. Hydrogel ultrafiltration appears to be an attractive alternative for the concentration of rotavirus and recombinant proteins from E. coli.

Original language	English
Pages (from-to)	224-225
Number of pages	2
Journal	Biotechnology and Bioprocess Engineering
Volume	4
Issue number	3
DOIs	https://doi.org/10.1007/BF02931934
Publication status	Published - 1999

Bibliographical note

Funding Information:
Acknowledgements This work was supported by a grant from the Academic Research Promotion Fund (Biochemical Engineering/1998-020-E00028) from Ministry of Education of Republic of Korea.

Keywords

Recombinant enhanced green fluorescent protein
Rotavirus
Temperature-sensitive hydrogel
Ultrafiltration

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10.1007/BF02931934

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title = "Concentrating rotavirus and recombinant enhanced green fluorescent protein from E. coli using a temperature-sensitive hydrogel",

abstract = "Recombinant enhanced green fluorescent protein (EGFP) from E. coli was concentrated 1.9 times by ultrafiltration using a temperature-sensitive hydrogel. Protein recovery and separation efficiency were 64\% and 45\%, respectively. Increased concentration of recombinant EGFP was confirmed by SDS-PAGE. Rotavirus was concentrated 3.2 times by ultrafiltration using a temperature-sensitive hydrogel, at 95\% of virus recovery and 93\% of separation efficiency. Hydrogel ultrafiltration appears to be an attractive alternative for the concentration of rotavirus and recombinant proteins from E. coli.",

keywords = "Recombinant enhanced green fluorescent protein, Rotavirus, Temperature-sensitive hydrogel, Ultrafiltration",

author = "Chang, \{Kynng Hwa\} and Park, \{Jong Hwa\} and Park, \{Chang Ho\} and Chung, \{In Sik\}",

note = "Funding Information: Acknowledgements This work was supported by a grant from the Academic Research Promotion Fund (Biochemical Engineering/1998-020-E00028) from Ministry of Education of Republic of Korea.",

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doi = "10.1007/BF02931934",

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T1 - Concentrating rotavirus and recombinant enhanced green fluorescent protein from E. coli using a temperature-sensitive hydrogel

AU - Chang, Kynng Hwa

AU - Park, Jong Hwa

AU - Park, Chang Ho

AU - Chung, In Sik

N1 - Funding Information: Acknowledgements This work was supported by a grant from the Academic Research Promotion Fund (Biochemical Engineering/1998-020-E00028) from Ministry of Education of Republic of Korea.

PY - 1999

Y1 - 1999

N2 - Recombinant enhanced green fluorescent protein (EGFP) from E. coli was concentrated 1.9 times by ultrafiltration using a temperature-sensitive hydrogel. Protein recovery and separation efficiency were 64% and 45%, respectively. Increased concentration of recombinant EGFP was confirmed by SDS-PAGE. Rotavirus was concentrated 3.2 times by ultrafiltration using a temperature-sensitive hydrogel, at 95% of virus recovery and 93% of separation efficiency. Hydrogel ultrafiltration appears to be an attractive alternative for the concentration of rotavirus and recombinant proteins from E. coli.

AB - Recombinant enhanced green fluorescent protein (EGFP) from E. coli was concentrated 1.9 times by ultrafiltration using a temperature-sensitive hydrogel. Protein recovery and separation efficiency were 64% and 45%, respectively. Increased concentration of recombinant EGFP was confirmed by SDS-PAGE. Rotavirus was concentrated 3.2 times by ultrafiltration using a temperature-sensitive hydrogel, at 95% of virus recovery and 93% of separation efficiency. Hydrogel ultrafiltration appears to be an attractive alternative for the concentration of rotavirus and recombinant proteins from E. coli.

KW - Recombinant enhanced green fluorescent protein

KW - Rotavirus

KW - Temperature-sensitive hydrogel

KW - Ultrafiltration

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DO - 10.1007/BF02931934

M3 - Article

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SN - 1226-8372

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SP - 224

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JO - Biotechnology and Bioprocess Engineering

JF - Biotechnology and Bioprocess Engineering

IS - 3

ER -

Concentrating rotavirus and recombinant enhanced green fluorescent protein from E. coli using a temperature-sensitive hydrogel

Abstract

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Keywords

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