Daidzein regulates secretion, production and gene expression of mucin from airway epithelial cells stimulated by proinflammatory factor and growth factor

Hyun Jae Lee, Su Yel Lee, Mi Nam Lee, Jang Hyun Kim, Gyu Tae Chang, Jeong Ho Seok, Choong Jae Lee

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

In this study, we investigated whether daidzein significantly affects secretion, production and gene expression of mucin from cultured airway epithelial cells. Confluent primary rat tracheal surface epithelial (RTSE) cells were pretreated with adenosine triphosphate (ATP) for 5 min and then chased for 30 min in the presence of daidzein to assess the effect on mucin secretion using ELISA. At the same time, confluent NCI-H292 cells were pretreated with daidzein for 30 min and then stimulated with EGF and PMA for 24 h, respectively. The MUC5AC mucin gene expression and mucin protein production were measured by RT-PCR and ELISA. The results were as follows: (1) daidzein significantly decreased ATP-induced mucin secretion from cultured RTSE cells; (2) daidzein inhibited the production of MUC5AC mucin protein induced by EGF or PMA from NCI-H292 cells; (3) daidzein also inhibited the expression of MUC5AC mucin gene induced by EGF or PMA from NCI-H292 cells. This result suggests that daidzein can regulate secretion, production and gene expression of mucin, by directly acting on airway epithelial cells.

Original languageEnglish
Pages (from-to)128-132
Number of pages5
JournalPulmonary Pharmacology and Therapeutics
Volume24
Issue number1
DOIs
Publication statusPublished - Feb 2011

Bibliographical note

Funding Information:
This study was partly supported by 2009 grant of Technology Development Program for Agriculture and Forestry, Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea .

Keywords

  • Airway
  • Daidzein
  • MUC5AC
  • Mucin

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