TY - JOUR
T1 - Description of Polaribacter batillariae sp. nov., Polaribacter cellanae sp. nov., and Polaribacter pectinis sp. nov., novel bacteria isolated from the gut of three types of South Korean shellfish
AU - Jeong, Su Won
AU - Han, Jeong Eun
AU - Lee, June Young
AU - Yoo, Ji Ho
AU - Kim, Do Yeon
AU - Jeong, In Chul
AU - Choi, Jee Won
AU - Jeong, Yun Seok
AU - Lee, Jae Yun
AU - Lee, So Yeon
AU - Tak, Euon Jung
AU - Sung, Hojun
AU - Kim, Hyun Sik
AU - Kim, Pil Soo
AU - Hyun, Dong Wook
AU - Bae, Jin Woo
N1 - Funding Information:
We thank Dr. Aharon Oren (The Herbrew University of Jerusalem, Israel) for etymological advice. This work was supported by a grant from the Mid-Career Researcher Program (NRF-2020R1A2C3012797) through the National Research Foundation of Korea (NRF) and funded by the Korea government (MSIT) (NRF-2018R1A5A1025077); and the National Institute of Biological Resources (NIBR), funded by the Ministry of Environment (MOE) of the Republic of Korea (NIBR202002108).
Publisher Copyright:
© 2022, Author(s).
PY - 2022/6
Y1 - 2022/6
N2 - Three aerobic, Gram-negative, and rod-shaped bacterial strains, designated strains G4M1T, SM13T, and L12M9T, were isolated from the gut of Batillaria multiformis, Cellana toreuma, and Patinopecten yessoensis collected from the Yellow Sea in South Korea. All the strains grew optimally at 25°C, in the presence of 2% (w/v) NaCl, and at pH 7. These three strains, which belonged to the genus Polaribacter in the family Flavobacteriaceae, shared < 98.8% in 16S rRNA gene sequence and < 86.68% in whole-genome sequence with each other. Compared with the type strains of Polaribacter, isolates showed the highest sequence similarity to P. haliotis KCTC 52418T (< 98.68%), followed by P. litorisediminis KCTC 52500T (< 98.13%). All the strains contained MK-6 as their predominant menaquinone and iso-C15:0 as their major fatty acid. Moreover, all the strains had phosphatidylethanolamine as their polar lipid component. In addition, strain G4M1T had two unidentified lipids and three unidentified aminolipids, strain SM13T had three unidentified lipids and three unidentified aminolipids, and strain L12M9T had three unidentified lipids and one unidentified aminolipid. The DNA G + C contents of strains G4M1T, SM13T, and L12M9T were 31.0, 30.4, and 29.7 mol%, respectively. Based on phenotypic, phylogenetic, chemotaxonomic, and genotypic findings, strains G4M1T (= KCTC 82388T = DSM 112372T), SM13T (= KCTC 82389T = DSM 112373T), and L12M9T (= KCTC 62751T = DSM 112374T) were classified into the genus Polaribacter as the type strains of novel species, for which the names Polaribacter batillariae sp. nov., Polaribacter cellanae sp. nov., and Polaribacter pectinis sp. nov., respectively, have been proposed.
AB - Three aerobic, Gram-negative, and rod-shaped bacterial strains, designated strains G4M1T, SM13T, and L12M9T, were isolated from the gut of Batillaria multiformis, Cellana toreuma, and Patinopecten yessoensis collected from the Yellow Sea in South Korea. All the strains grew optimally at 25°C, in the presence of 2% (w/v) NaCl, and at pH 7. These three strains, which belonged to the genus Polaribacter in the family Flavobacteriaceae, shared < 98.8% in 16S rRNA gene sequence and < 86.68% in whole-genome sequence with each other. Compared with the type strains of Polaribacter, isolates showed the highest sequence similarity to P. haliotis KCTC 52418T (< 98.68%), followed by P. litorisediminis KCTC 52500T (< 98.13%). All the strains contained MK-6 as their predominant menaquinone and iso-C15:0 as their major fatty acid. Moreover, all the strains had phosphatidylethanolamine as their polar lipid component. In addition, strain G4M1T had two unidentified lipids and three unidentified aminolipids, strain SM13T had three unidentified lipids and three unidentified aminolipids, and strain L12M9T had three unidentified lipids and one unidentified aminolipid. The DNA G + C contents of strains G4M1T, SM13T, and L12M9T were 31.0, 30.4, and 29.7 mol%, respectively. Based on phenotypic, phylogenetic, chemotaxonomic, and genotypic findings, strains G4M1T (= KCTC 82388T = DSM 112372T), SM13T (= KCTC 82389T = DSM 112373T), and L12M9T (= KCTC 62751T = DSM 112374T) were classified into the genus Polaribacter as the type strains of novel species, for which the names Polaribacter batillariae sp. nov., Polaribacter cellanae sp. nov., and Polaribacter pectinis sp. nov., respectively, have been proposed.
KW - Batillaria multiformis
KW - Cellana toreuma
KW - gut microbiota
KW - Patinopecten yessoensis
KW - Polaribacter batillariae
KW - Polaribacter cellanae
KW - Polaribacter pectinis
UR - http://www.scopus.com/inward/record.url?scp=85128419215&partnerID=8YFLogxK
U2 - 10.1007/s12275-022-1604-3
DO - 10.1007/s12275-022-1604-3
M3 - Article
C2 - 35437627
AN - SCOPUS:85128419215
VL - 60
SP - 576
EP - 584
JO - Journal of Microbiology
JF - Journal of Microbiology
SN - 1225-8873
IS - 6
ER -