Detection and quantification of the Bcr/Abl chimeric protein on biochips using LDI-TOF MS

Seol Hye Hong; Jae Il Kim; Hyunook Kang; Soojin Yoon; Dong Eun Kim; Woong Jung; Woon Seok Yeo

doi:10.1039/c4cc01332h

Detection and quantification of the Bcr/Abl chimeric protein on biochips using LDI-TOF MS

Seol Hye Hong
, Jae Il Kim
, Hyunook Kang
, Soojin Yoon
, Dong Eun Kim
, Woong Jung
, Woon Seok Yeo

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

The Bcr/Abl chimeric protein was captured by two antibodies, anti-Bcr on gold nanoparticles (AuNPs) and anti-Abl on a biochip, in a sandwich assay format. The presence of the Bcr/Abl in cells was then verified by amplified LDI-TOF MS signals, and relative amounts were quantified using AuNPs coated with deuterated alkanethiols as an internal standard.

Original language	English
Pages (from-to)	4831-4834
Number of pages	4
Journal	Chemical Communications
Volume	50
Issue number	37
DOIs	https://doi.org/10.1039/c4cc01332h
Publication status	Published - 10 Apr 2014

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abstract = "The Bcr/Abl chimeric protein was captured by two antibodies, anti-Bcr on gold nanoparticles (AuNPs) and anti-Abl on a biochip, in a sandwich assay format. The presence of the Bcr/Abl in cells was then verified by amplified LDI-TOF MS signals, and relative amounts were quantified using AuNPs coated with deuterated alkanethiols as an internal standard.",

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AU - Hong, Seol Hye

AU - Kim, Jae Il

AU - Kang, Hyunook

AU - Yoon, Soojin

AU - Kim, Dong Eun

AU - Jung, Woong

AU - Yeo, Woon Seok

PY - 2014/4/10

Y1 - 2014/4/10

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AB - The Bcr/Abl chimeric protein was captured by two antibodies, anti-Bcr on gold nanoparticles (AuNPs) and anti-Abl on a biochip, in a sandwich assay format. The presence of the Bcr/Abl in cells was then verified by amplified LDI-TOF MS signals, and relative amounts were quantified using AuNPs coated with deuterated alkanethiols as an internal standard.

UR - https://www.scopus.com/pages/publications/84898627410

U2 - 10.1039/c4cc01332h

DO - 10.1039/c4cc01332h

M3 - Article

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AN - SCOPUS:84898627410

SN - 1359-7345

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SP - 4831

EP - 4834

JO - Chemical Communications

JF - Chemical Communications

IS - 37

ER -

Detection and quantification of the Bcr/Abl chimeric protein on biochips using LDI-TOF MS

Abstract

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