Determination of chloramphenicol residues in foods by ELISA and LC-MS/MS coupled with molecularly imprinted solid phase extraction

Xizhi Shi; Suquan Song; Aili Sun; Dexiang Li; Aibo Wu; Dabing Zhang

doi:10.1080/00032711003763616

Determination of chloramphenicol residues in foods by ELISA and LC-MS/MS coupled with molecularly imprinted solid phase extraction

Xizhi Shi
, Suquan Song
, Aili Sun
, Dexiang Li
, Aibo Wu
, Dabing Zhang

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

The combination of molecularly imprinted solid-phase extraction (MISPE) with ELISA and LC-MS/MS was developed for the detection of chloramphenicol (CAP) in honey samples. Significant recoveries of 99.1±7.1 and 98.8±8.2% were obtained for intraand inter-assay determination by ELISA determination, respectively. The limit of detection of CAP was 0.034 μgkg^-1 and the limit of quantification was 0.046 μgkg^-1. Determination and validation of CAP by using LC-MS/MS were performed following the same extraction and purification process as for the ELISA. The results demonstrated that the CAP samples purified by using MISPE were simultaneously applicable to analysis by ELISA and LC-MS/MS.

Original language	English
Pages (from-to)	2798-2807
Number of pages	10
Journal	Analytical Letters
Volume	43
Issue number	17
DOIs	https://doi.org/10.1080/00032711003763616
Publication status	Published - Jan 2010

Bibliographical note

Funding Information:
Received 7 September 2009; accepted 9 November 2009. These authors contributed equally to this work. This work was supported by the A Project Supported by Scientific Research Fund of Zhejiang Provincial Education Department (Y200803423), Natural Science Foundation of Ningbo (2009A610146), National ‘‘863’’ High-Tech Project (2006AA10Z438), the National Key Project of Scientific and Technical Supporting Programs Funded by Ministry of Science & Technology of China (2006BAK02A09), and Scientific Research Fund of Ningbo University (XYL08009). Address correspondence to Xizhi Shi, Faculty of Life Science and Biotechnology, Ningbo University, 818 Fenghua Road, Ningbo 315211, China. E-mail: [email protected]

Keywords

Chloramphenicol
Foods
Molecularly imprinted polymer microspheres

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10.1080/00032711003763616

Cite this

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title = "Determination of chloramphenicol residues in foods by ELISA and LC-MS/MS coupled with molecularly imprinted solid phase extraction",

abstract = "The combination of molecularly imprinted solid-phase extraction (MISPE) with ELISA and LC-MS/MS was developed for the detection of chloramphenicol (CAP) in honey samples. Significant recoveries of 99.1±7.1 and 98.8±8.2\% were obtained for intraand inter-assay determination by ELISA determination, respectively. The limit of detection of CAP was 0.034 μgkg-1 and the limit of quantification was 0.046 μgkg-1. Determination and validation of CAP by using LC-MS/MS were performed following the same extraction and purification process as for the ELISA. The results demonstrated that the CAP samples purified by using MISPE were simultaneously applicable to analysis by ELISA and LC-MS/MS.",

keywords = "Chloramphenicol, Foods, Molecularly imprinted polymer microspheres",

author = "Xizhi Shi and Suquan Song and Aili Sun and Dexiang Li and Aibo Wu and Dabing Zhang",

note = "Funding Information: Received 7 September 2009; accepted 9 November 2009. These authors contributed equally to this work. This work was supported by the A Project Supported by Scientific Research Fund of Zhejiang Provincial Education Department (Y200803423), Natural Science Foundation of Ningbo (2009A610146), National {\textquoteleft}{\textquoteleft}863{\textquoteright}{\textquoteright} High-Tech Project (2006AA10Z438), the National Key Project of Scientific and Technical Supporting Programs Funded by Ministry of Science \& Technology of China (2006BAK02A09), and Scientific Research Fund of Ningbo University (XYL08009). Address correspondence to Xizhi Shi, Faculty of Life Science and Biotechnology, Ningbo University, 818 Fenghua Road, Ningbo 315211, China. E-mail: [email protected]",

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doi = "10.1080/00032711003763616",

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volume = "43",

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TY - JOUR

T1 - Determination of chloramphenicol residues in foods by ELISA and LC-MS/MS coupled with molecularly imprinted solid phase extraction

AU - Shi, Xizhi

AU - Song, Suquan

AU - Sun, Aili

AU - Li, Dexiang

AU - Wu, Aibo

AU - Zhang, Dabing

N1 - Funding Information: Received 7 September 2009; accepted 9 November 2009. These authors contributed equally to this work. This work was supported by the A Project Supported by Scientific Research Fund of Zhejiang Provincial Education Department (Y200803423), Natural Science Foundation of Ningbo (2009A610146), National ‘‘863’’ High-Tech Project (2006AA10Z438), the National Key Project of Scientific and Technical Supporting Programs Funded by Ministry of Science & Technology of China (2006BAK02A09), and Scientific Research Fund of Ningbo University (XYL08009). Address correspondence to Xizhi Shi, Faculty of Life Science and Biotechnology, Ningbo University, 818 Fenghua Road, Ningbo 315211, China. E-mail: [email protected]

PY - 2010/1

Y1 - 2010/1

N2 - The combination of molecularly imprinted solid-phase extraction (MISPE) with ELISA and LC-MS/MS was developed for the detection of chloramphenicol (CAP) in honey samples. Significant recoveries of 99.1±7.1 and 98.8±8.2% were obtained for intraand inter-assay determination by ELISA determination, respectively. The limit of detection of CAP was 0.034 μgkg-1 and the limit of quantification was 0.046 μgkg-1. Determination and validation of CAP by using LC-MS/MS were performed following the same extraction and purification process as for the ELISA. The results demonstrated that the CAP samples purified by using MISPE were simultaneously applicable to analysis by ELISA and LC-MS/MS.

AB - The combination of molecularly imprinted solid-phase extraction (MISPE) with ELISA and LC-MS/MS was developed for the detection of chloramphenicol (CAP) in honey samples. Significant recoveries of 99.1±7.1 and 98.8±8.2% were obtained for intraand inter-assay determination by ELISA determination, respectively. The limit of detection of CAP was 0.034 μgkg-1 and the limit of quantification was 0.046 μgkg-1. Determination and validation of CAP by using LC-MS/MS were performed following the same extraction and purification process as for the ELISA. The results demonstrated that the CAP samples purified by using MISPE were simultaneously applicable to analysis by ELISA and LC-MS/MS.

KW - Chloramphenicol

KW - Foods

KW - Molecularly imprinted polymer microspheres

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DO - 10.1080/00032711003763616

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SN - 0003-2719

VL - 43

SP - 2798

EP - 2807

JO - Analytical Letters

JF - Analytical Letters

IS - 17

ER -

Determination of chloramphenicol residues in foods by ELISA and LC-MS/MS coupled with molecularly imprinted solid phase extraction

Abstract

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