Development of a rapid and accurate identification method for citrobacter species isolated from pork products using a matrix-assisted laser-desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS)

Hye Lim Kwak; Sun Kyung Han; Sunghoon Park; Si Hong Park; Jae Yong Shim; Mihwa Oh; Steven C. Ricke; Hae Yeong Kim

doi:10.4014/jmb.1503.03071

Development of a rapid and accurate identification method for citrobacter species isolated from pork products using a matrix-assisted laser-desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS)

Hye Lim Kwak
, Sun Kyung Han
, Sunghoon Park
, Si Hong Park
, Jae Yong Shim
, Mihwa Oh
, Steven C. Ricke
, Hae Yeong Kim

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

Previous detection methods for Citrobacter are considered time consuming and laborious. In this study, we have developed a rapid and accurate detection method for Citrobacter species in pork products, using matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS). A total of 35 Citrobacter strains were isolated from 30 pork products and identified by both MALDI-TOF MS and 16S rRNA gene sequencing approaches. All isolates were identified to the species level by the MALDI-TOF MS, while 16S rRNA gene sequencing results could not discriminate them clearly. These results confirmed that MALDITOF MS is a more accurate and rapid detection method for the identification of Citrobacter species.

Original language	English
Pages (from-to)	1537-1541
Number of pages	5
Journal	Journal of Microbiology and Biotechnology
Volume	25
Issue number	9
DOIs	https://doi.org/10.4014/jmb.1503.03071
Publication status	Published - 26 May 2015

Bibliographical note

Publisher Copyright:
© 2015 by The Korean Society for Microbiology and Biotechnology.

Keywords

Citrobacter species
Identification
MALDI-TOF MS
Pork products

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Kwak, H. L., Han, S. K., Park, S., Park, S. H., Shim, J. Y., Oh, M., Ricke, S. C., & Kim, H. Y. (2015). Development of a rapid and accurate identification method for citrobacter species isolated from pork products using a matrix-assisted laser-desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). Journal of Microbiology and Biotechnology, 25(9), 1537-1541. https://doi.org/10.4014/jmb.1503.03071

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title = "Development of a rapid and accurate identification method for citrobacter species isolated from pork products using a matrix-assisted laser-desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS)",

abstract = "Previous detection methods for Citrobacter are considered time consuming and laborious. In this study, we have developed a rapid and accurate detection method for Citrobacter species in pork products, using matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS). A total of 35 Citrobacter strains were isolated from 30 pork products and identified by both MALDI-TOF MS and 16S rRNA gene sequencing approaches. All isolates were identified to the species level by the MALDI-TOF MS, while 16S rRNA gene sequencing results could not discriminate them clearly. These results confirmed that MALDITOF MS is a more accurate and rapid detection method for the identification of Citrobacter species.",

keywords = "Citrobacter species, Identification, MALDI-TOF MS, Pork products",

author = "Kwak, \{Hye Lim\} and Han, \{Sun Kyung\} and Sunghoon Park and Park, \{Si Hong\} and Shim, \{Jae Yong\} and Mihwa Oh and Ricke, \{Steven C.\} and Kim, \{Hae Yeong\}",

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year = "2015",

month = may,

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doi = "10.4014/jmb.1503.03071",

language = "English",

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Kwak, HL, Han, SK, Park, S, Park, SH, Shim, JY, Oh, M, Ricke, SC & Kim, HY 2015, 'Development of a rapid and accurate identification method for citrobacter species isolated from pork products using a matrix-assisted laser-desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS)', Journal of Microbiology and Biotechnology, vol. 25, no. 9, pp. 1537-1541. https://doi.org/10.4014/jmb.1503.03071

Development of a rapid and accurate identification method for citrobacter species isolated from pork products using a matrix-assisted laser-desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). / Kwak, Hye Lim; Han, Sun Kyung; Park, Sunghoon et al.
In: Journal of Microbiology and Biotechnology, Vol. 25, No. 9, 26.05.2015, p. 1537-1541.

Research output: Contribution to journal › Article › peer-review

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T1 - Development of a rapid and accurate identification method for citrobacter species isolated from pork products using a matrix-assisted laser-desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS)

AU - Kwak, Hye Lim

AU - Han, Sun Kyung

AU - Park, Sunghoon

AU - Park, Si Hong

AU - Shim, Jae Yong

AU - Oh, Mihwa

AU - Ricke, Steven C.

AU - Kim, Hae Yeong

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N2 - Previous detection methods for Citrobacter are considered time consuming and laborious. In this study, we have developed a rapid and accurate detection method for Citrobacter species in pork products, using matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS). A total of 35 Citrobacter strains were isolated from 30 pork products and identified by both MALDI-TOF MS and 16S rRNA gene sequencing approaches. All isolates were identified to the species level by the MALDI-TOF MS, while 16S rRNA gene sequencing results could not discriminate them clearly. These results confirmed that MALDITOF MS is a more accurate and rapid detection method for the identification of Citrobacter species.

AB - Previous detection methods for Citrobacter are considered time consuming and laborious. In this study, we have developed a rapid and accurate detection method for Citrobacter species in pork products, using matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS). A total of 35 Citrobacter strains were isolated from 30 pork products and identified by both MALDI-TOF MS and 16S rRNA gene sequencing approaches. All isolates were identified to the species level by the MALDI-TOF MS, while 16S rRNA gene sequencing results could not discriminate them clearly. These results confirmed that MALDITOF MS is a more accurate and rapid detection method for the identification of Citrobacter species.

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KW - Identification

KW - MALDI-TOF MS

KW - Pork products

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ER -

Development of a rapid and accurate identification method for citrobacter species isolated from pork products using a matrix-assisted laser-desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS)

Abstract

Bibliographical note

Keywords

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