Abstract
The central goal of current genomics research in plants, as in other organisms, is to elucidate the functions of every gene. Insertional mutagenesis using known DNA sequences such as T-DNA is a powerful tool in functional genomics. Development of efficient methods for isolating the genomic sequences flanking insertion elements accelerates the systematic cataloging of insertional mutants, and thus allows functions to be assigned to uncharacterized genes via reverse genetic approaches. In our current study, we report a rapid and efficient inverse PCR (iPCR) method for the isolation of gene tags in T-DNA mutant lines of rice (Oryza sativa), a model monocot plant.
Original language | English |
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Title of host publication | Methods in Molecular Biology |
Publisher | Humana Press Inc. |
Pages | 139-146 |
Number of pages | 8 |
DOIs | |
Publication status | Published - 2011 |
Publication series
Name | Methods in Molecular Biology |
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Volume | 678 |
ISSN (Print) | 1064-3745 |
ISSN (Electronic) | 1940-6029 |
Bibliographical note
Publisher Copyright:© 2011, Springer Science+Business Media, LLC.
Keywords
- Functional genomics
- Gene tag
- Inverse PCR
- Rice