Differential gene expression profiles of periodontal soft tissue from rat teeth after immediate and delayed replantation: a pilot study

Yong Kwon Chae, Seo Young Shin, Sang Wook Kang, Sung Chul Choi, Ok Hyung Nam

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Purpose: In dental avulsion, delayed replantation usually has an uncertain prognosis. After tooth replantation, complex inflammatory responses promote a return to periodontal tissue homeostasis. Various types of cytokines are produced in the inflammatory microenvironment, and these cytokines determine the periodontal tissue response. This study aimed to identify the gene expression profiles of replanted teeth and evaluate the functional differences between immediate and delayed replantation. Methods: Maxillary molars from Sprague-Dawley rats were extracted, exposed to a dry environment, and then replanted. The animals were divided into 2 groups according to the extra-oral time: immediate replantation (dry for 5 minutes) and delayed replantation (dry for 60 minutes). Either 3 or 7 days after replantation, the animals were sacrificed. Periodontal soft tissues were harvested for mRNA sequencing. Hallmark gene set enrichment analysis was performed to predict the function of gene-gene interactions. The normalized enrichment score (NES) was calculated to determine functional differences. Results: The hallmark gene sets enriched in delayed replantation at 3 days were oxidative phosphorylation (NES=2.82, Q<0.001) and tumor necrosis factor-alpha (TNF-α) signaling via the nuclear factor kappa light chain enhancer of activated B cells (NF-κB) pathway (NES=1.52, Q=0.034). At 7 days after delayed replantation, TNF-α signaling via the NF-κB pathway (NES=–1.82, Q=0.002), angiogenesis (NES=–1.66, Q=0.01), and the transforming growth factor-beta signaling pathway (NES=–1.46, Q=0.051) were negatively highlighted. Conclusions: Differentially expressed gene profiles were significantly different between immediate and delayed replantation. TNF-α signaling via the NF-κB pathway was marked during the healing process. However, the enrichment score of this pathway changed in a time-dependent manner between immediate and delayed replantation.

Original languageEnglish
Article numbere45
JournalJournal of Periodontal and Implant Science
Volume51
Issue number6
DOIs
Publication statusPublished - 2021

Bibliographical note

Publisher Copyright:
© 2021. Korean Academy of Periodontology This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/).

Keywords

  • Gene expression profiling
  • Rna sequencing
  • Tooth avulsion
  • Tooth replantation

Fingerprint

Dive into the research topics of 'Differential gene expression profiles of periodontal soft tissue from rat teeth after immediate and delayed replantation: a pilot study'. Together they form a unique fingerprint.

Cite this