Differential modulation of AMPK signaling pathways by low or high levels of exogenous reactive oxygen species in colon cancer cells

In Ja Park; Jin Taek Hwang; Min Kim Young; Joohun Ha; Jin Park Ock

doi:10.1196/annals.1378.059

Differential modulation of AMPK signaling pathways by low or high levels of exogenous reactive oxygen species in colon cancer cells

In Ja Park, Jin Taek Hwang, Min Kim Young, Joohun Ha, Jin Park Ock

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

63 Citations (Scopus)

Abstract

This study was undertaken to examine the effect of low and high concentrations of H₂O₂ on cancer cell proliferation and apoptosis, and AMPK signaling pathways in HT-29 human colon cancer cells. Nontoxic doses of H₂O₂ (10 μM) induced cancer cell proliferation, whereas the toxic level of 1,000 μM H₂O₂ induced apoptosis. The stimulation of cell proliferation was accompanied with an increase in cyclooxygenase-2 (COX-2), and apoptosis induced by high-dose H₂O₂ was correlated with the activation of AMPK and negatively correlated with COX-2 expression. These results suggest that ROS at nontoxic levels can stimulate cancer cell growth by regulating AMP-activated protein kinase (AMPK) and/or COX-2, and the abundant exogenous ROS linked to the growth inhibition through modulating AMPK signaling pathways.

Original language	English
Title of host publication	Signal Transduction Pathways, Part B
Subtitle of host publication	Stress Signalling and Transcriptional Control
Publisher	Blackwell Publishing Inc.
Pages	102-109
Number of pages	8
ISBN (Print)	1573316474, 9781573316477
DOIs	https://doi.org/10.1196/annals.1378.059
Publication status	Published - Dec 2006

Publication series

Name	Annals of the New York Academy of Sciences
Volume	1091
ISSN (Print)	0077-8923
ISSN (Electronic)	1749-6632

Keywords

AMP-activated protein kinase
Cyclooxygenase-2
HT-29 colon cancer cells
High-dose HO
Low-dose HO
Reactive oxygen species

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1196/annals.1378.059

Cite this

Park, I. J., Hwang, J. T., Young, M. K., Ha, J., & Ock, J. P. (2006). Differential modulation of AMPK signaling pathways by low or high levels of exogenous reactive oxygen species in colon cancer cells. In Signal Transduction Pathways, Part B: Stress Signalling and Transcriptional Control (pp. 102-109). (Annals of the New York Academy of Sciences; Vol. 1091). Blackwell Publishing Inc.. https://doi.org/10.1196/annals.1378.059

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title = "Differential modulation of AMPK signaling pathways by low or high levels of exogenous reactive oxygen species in colon cancer cells",

abstract = "This study was undertaken to examine the effect of low and high concentrations of H2O2 on cancer cell proliferation and apoptosis, and AMPK signaling pathways in HT-29 human colon cancer cells. Nontoxic doses of H2O2 (10 μM) induced cancer cell proliferation, whereas the toxic level of 1,000 μM H2O2 induced apoptosis. The stimulation of cell proliferation was accompanied with an increase in cyclooxygenase-2 (COX-2), and apoptosis induced by high-dose H2O2 was correlated with the activation of AMPK and negatively correlated with COX-2 expression. These results suggest that ROS at nontoxic levels can stimulate cancer cell growth by regulating AMP-activated protein kinase (AMPK) and/or COX-2, and the abundant exogenous ROS linked to the growth inhibition through modulating AMPK signaling pathways.",

keywords = "AMP-activated protein kinase, Cyclooxygenase-2, HT-29 colon cancer cells, High-dose HO, Low-dose HO, Reactive oxygen species",

author = "Park, {In Ja} and Hwang, {Jin Taek} and Young, {Min Kim} and Joohun Ha and Ock, {Jin Park}",

year = "2006",

month = dec,

doi = "10.1196/annals.1378.059",

language = "English",

isbn = "1573316474",

series = "Annals of the New York Academy of Sciences",

publisher = "Blackwell Publishing Inc.",

pages = "102--109",

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Park, IJ, Hwang, JT, Young, MK, Ha, J & Ock, JP 2006, Differential modulation of AMPK signaling pathways by low or high levels of exogenous reactive oxygen species in colon cancer cells. in Signal Transduction Pathways, Part B: Stress Signalling and Transcriptional Control. Annals of the New York Academy of Sciences, vol. 1091, Blackwell Publishing Inc., pp. 102-109. https://doi.org/10.1196/annals.1378.059

Differential modulation of AMPK signaling pathways by low or high levels of exogenous reactive oxygen species in colon cancer cells. / Park, In Ja; Hwang, Jin Taek; Young, Min Kim et al.
Signal Transduction Pathways, Part B: Stress Signalling and Transcriptional Control. Blackwell Publishing Inc., 2006. p. 102-109 (Annals of the New York Academy of Sciences; Vol. 1091).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Differential modulation of AMPK signaling pathways by low or high levels of exogenous reactive oxygen species in colon cancer cells

AU - Park, In Ja

AU - Hwang, Jin Taek

AU - Young, Min Kim

AU - Ha, Joohun

AU - Ock, Jin Park

PY - 2006/12

Y1 - 2006/12

N2 - This study was undertaken to examine the effect of low and high concentrations of H2O2 on cancer cell proliferation and apoptosis, and AMPK signaling pathways in HT-29 human colon cancer cells. Nontoxic doses of H2O2 (10 μM) induced cancer cell proliferation, whereas the toxic level of 1,000 μM H2O2 induced apoptosis. The stimulation of cell proliferation was accompanied with an increase in cyclooxygenase-2 (COX-2), and apoptosis induced by high-dose H2O2 was correlated with the activation of AMPK and negatively correlated with COX-2 expression. These results suggest that ROS at nontoxic levels can stimulate cancer cell growth by regulating AMP-activated protein kinase (AMPK) and/or COX-2, and the abundant exogenous ROS linked to the growth inhibition through modulating AMPK signaling pathways.

AB - This study was undertaken to examine the effect of low and high concentrations of H2O2 on cancer cell proliferation and apoptosis, and AMPK signaling pathways in HT-29 human colon cancer cells. Nontoxic doses of H2O2 (10 μM) induced cancer cell proliferation, whereas the toxic level of 1,000 μM H2O2 induced apoptosis. The stimulation of cell proliferation was accompanied with an increase in cyclooxygenase-2 (COX-2), and apoptosis induced by high-dose H2O2 was correlated with the activation of AMPK and negatively correlated with COX-2 expression. These results suggest that ROS at nontoxic levels can stimulate cancer cell growth by regulating AMP-activated protein kinase (AMPK) and/or COX-2, and the abundant exogenous ROS linked to the growth inhibition through modulating AMPK signaling pathways.

KW - AMP-activated protein kinase

KW - Cyclooxygenase-2

KW - HT-29 colon cancer cells

KW - High-dose HO

KW - Low-dose HO

KW - Reactive oxygen species

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U2 - 10.1196/annals.1378.059

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M3 - Conference contribution

C2 - 17341607

AN - SCOPUS:33947602679

SN - 1573316474

SN - 9781573316477

T3 - Annals of the New York Academy of Sciences

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BT - Signal Transduction Pathways, Part B

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ER -

Park IJ, Hwang JT, Young MK, Ha J, Ock JP. Differential modulation of AMPK signaling pathways by low or high levels of exogenous reactive oxygen species in colon cancer cells. In Signal Transduction Pathways, Part B: Stress Signalling and Transcriptional Control. Blackwell Publishing Inc. 2006. p. 102-109. (Annals of the New York Academy of Sciences). doi: 10.1196/annals.1378.059

Differential modulation of AMPK signaling pathways by low or high levels of exogenous reactive oxygen species in colon cancer cells

Abstract

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Keywords

UN SDGs

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