Droplet-based biogenic paramagnetic nanoparticle synthesis

J. H. Jung, T. J. Park, Y. Piao, S. Y. Lee, T. S. Seo

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Droplet-based microfluidic system for biogenic paramagnetic nanoparticle synthesis is reported in this study. The engineered recombinant Escherichia coli (E. coli), which expressed heavy metal-binding proteins, were injected to droplets in the microchannel together with iron and manganese solution. Homogeneous droplets were generated and went through a serpentine channel to mix the three components efficiently. The droplets were collected from the outlet and incubated overnight at 37 °C. The synthesis of Fe-Mn paramagnetic nanoparticle inside E. coli was confirmed by detecting the movement of cells in the droplets induced by an external magnet, and was characterized by TEM.

Original languageEnglish
Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages879-881
Number of pages3
Publication statusPublished - 2011
Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
Duration: 2 Oct 20116 Oct 2011

Publication series

Name15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Volume2

Conference

Conference15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Country/TerritoryUnited States
CitySeattle, WA
Period2/10/116/10/11

Keywords

  • Biogenic nanoparticle
  • Droplet
  • Escherichia coli
  • Microfluidic

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