TY - JOUR
T1 - Effect of HT042, herbal formula, on longitudinal bone growth in spontaneous dwarf rats
AU - Kim, Ji Young
AU - Song, Mikyung
AU - Lee, Donghun
AU - Song, Jungbin
AU - Park, Sang Woug
AU - Park, Juyeon
AU - Park, Seungjoon
AU - Choi, Ho Young
AU - Kim, Hocheol
PY - 2013/11
Y1 - 2013/11
N2 - HT042 is a new herbal prescription consisting of Astragalus membranaceus, Phlomis umbrosa and Eleutherococcus senticosus, which are used in Korean medicine to stimulate growth in children. We investigated the effects of HT042 on the body weight, longitudinal bone growth, and bone length in spontaneous dwarf rats (SDR). Male and female SDRs were divided into three groups: the control group (DW, 10 mL/kg/day), the recombinant human GH group (rhGH; 500 μg/kg/day), and the HT042 (100 mg/kg/day) group. Each group received the respective treatments for 10 days. Body weight was measured on day 10 of treatment. On day 8, tetracycline (20 mg/kg) was injected intraperitoneally into all individuals to form a fluorescent band on the newly synthesized bone. On day 10, femur and tibia lengths were measured using PIXImus. Body weight, longitudinal bone growth, and bone length were not affected in the HT042 group. In contrast, the rhGH group showed significantly increased body weight, longitudinal bone growth, and bone length. In conclusion, HT042 does not act through a GH-like effect to promote longitudinal bone growth.
AB - HT042 is a new herbal prescription consisting of Astragalus membranaceus, Phlomis umbrosa and Eleutherococcus senticosus, which are used in Korean medicine to stimulate growth in children. We investigated the effects of HT042 on the body weight, longitudinal bone growth, and bone length in spontaneous dwarf rats (SDR). Male and female SDRs were divided into three groups: the control group (DW, 10 mL/kg/day), the recombinant human GH group (rhGH; 500 μg/kg/day), and the HT042 (100 mg/kg/day) group. Each group received the respective treatments for 10 days. Body weight was measured on day 10 of treatment. On day 8, tetracycline (20 mg/kg) was injected intraperitoneally into all individuals to form a fluorescent band on the newly synthesized bone. On day 10, femur and tibia lengths were measured using PIXImus. Body weight, longitudinal bone growth, and bone length were not affected in the HT042 group. In contrast, the rhGH group showed significantly increased body weight, longitudinal bone growth, and bone length. In conclusion, HT042 does not act through a GH-like effect to promote longitudinal bone growth.
KW - HT042
KW - Herbal extract
KW - Longitudinal bone growth
KW - Spontaneous dwarf rat
UR - http://www.scopus.com/inward/record.url?scp=84888590306&partnerID=8YFLogxK
U2 - 10.3390/molecules181113271
DO - 10.3390/molecules181113271
M3 - Article
C2 - 24169467
AN - SCOPUS:84888590306
SN - 1420-3049
VL - 18
SP - 13271
EP - 13282
JO - Molecules
JF - Molecules
IS - 11
ER -
