Abstract
Objective: The objective of the study was to evaluate the healing effect of hyaluronic acid films on palatal wounds. Materials and Methods: After making 5-mm diameter palatal wounds, 72 rats were randomly assigned to three groups: control, hyaluronic acid gel, and hyaluronic acid film. The animals were sacrificed at 3, 7, and 21 days after the experiment. Clinical, histological, and RT-PCR analyses were performed. Human ex vivo oral mucosa models were used. Histological analysis and pan-cytokeratin staining were performed at 5 days after wound creation. Results: In rat model, both gels and films showed favorable healing on Days 3 and 7 compared with healing in the control (p < 0.05). Film showed remarkable VEGF and α-SMA expression than did the others (p < 0.05). Immunohistochemical analysis showed that film exhibited significantly lower CD68 and greater α-SMA and vimentin expression levels than those in the others (p < 0.05). In human model, re-epithelialization rate of film group was significantly higher than that of the others. Complete epithelial regeneration was confirmed only in film group using pan-cytokeratin staining. Conclusions: Within the limits of this study, hyaluronic acid film outperformed gels in terms of palatal wound healing in both models.
| Original language | English |
|---|---|
| Pages (from-to) | 518-527 |
| Number of pages | 10 |
| Journal | Oral Diseases |
| Volume | 30 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Mar 2024 |
Bibliographical note
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Keywords
- drug vehicle
- gingiva
- hyaluronic acid
- wound healing