Effects of ß-glucans from Coriolus versicolor on macrophage phagocytosis are related to the Akt and CK2/Ikaros

Se Chan Kang; Hyun Jung Koo; Sulkyung Park; Jung Dae Lim; Ye Jin Kim; Taeseong Kim; Seung Namkoong; Ki Hyo Jang; Suhkneung Pyo; Seon A. Jang; Eun Hwa Sohn

doi:10.1016/j.ijbiomac.2013.03.017

Effects of ß-glucans from Coriolus versicolor on macrophage phagocytosis are related to the Akt and CK2/Ikaros

Se Chan Kang, Hyun Jung Koo, Sulkyung Park, Jung Dae Lim, Ye Jin Kim, Taeseong Kim, Seung Namkoong, Ki Hyo Jang, Suhkneung Pyo, Seon A. Jang, Eun Hwa Sohn

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

Coriolus versicolor has been known to be an immune stimulator effects. For further understanding of the phagocytic activity and the intracellular mechanisms of ß-glucan from C. versicolor (CVG), we examined the phagocytic activity, phosphorylation of Akt and CK2, nucleus translocation of p65 and Ikaros activity in ß-glucan-treated macrophages using RT-PCR, western blotting, and IP assay. The role of Ikaros in regulating phagocytic effects of CVG was also determined using Ikaros dominant negative isoform cells. This study suggests that CK2/Ikaros are positive regulators and novel signaling pathway involved in phagocytosis and contributes to elucidating the mechanism underlying phagocytic activity induced by ß-glucan.

Original language	English
Pages (from-to)	9-16
Number of pages	8
Journal	International Journal of Biological Macromolecules
Volume	57
DOIs	https://doi.org/10.1016/j.ijbiomac.2013.03.017
Publication status	Published - Jun 2013

Keywords

Beta-glucan
Coriolus versicolor
Dectin-1
Phagocytic activity
RAW264.7

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10.1016/j.ijbiomac.2013.03.017

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@article{35310f863a6c404290ea38451fb94919,

title = "Effects of {\ss}-glucans from Coriolus versicolor on macrophage phagocytosis are related to the Akt and CK2/Ikaros",

abstract = "Coriolus versicolor has been known to be an immune stimulator effects. For further understanding of the phagocytic activity and the intracellular mechanisms of {\ss}-glucan from C. versicolor (CVG), we examined the phagocytic activity, phosphorylation of Akt and CK2, nucleus translocation of p65 and Ikaros activity in {\ss}-glucan-treated macrophages using RT-PCR, western blotting, and IP assay. The role of Ikaros in regulating phagocytic effects of CVG was also determined using Ikaros dominant negative isoform cells. This study suggests that CK2/Ikaros are positive regulators and novel signaling pathway involved in phagocytosis and contributes to elucidating the mechanism underlying phagocytic activity induced by {\ss}-glucan.",

keywords = "Beta-glucan, Coriolus versicolor, Dectin-1, Phagocytic activity, RAW264.7",

author = "Kang, {Se Chan} and Koo, {Hyun Jung} and Sulkyung Park and Lim, {Jung Dae} and Kim, {Ye Jin} and Taeseong Kim and Seung Namkoong and Jang, {Ki Hyo} and Suhkneung Pyo and Jang, {Seon A.} and Sohn, {Eun Hwa}",

year = "2013",

month = jun,

doi = "10.1016/j.ijbiomac.2013.03.017",

language = "English",

volume = "57",

pages = "9--16",

journal = "International Journal of Biological Macromolecules",

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publisher = "Elsevier",

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T1 - Effects of ß-glucans from Coriolus versicolor on macrophage phagocytosis are related to the Akt and CK2/Ikaros

AU - Kang, Se Chan

AU - Koo, Hyun Jung

AU - Park, Sulkyung

AU - Lim, Jung Dae

AU - Kim, Ye Jin

AU - Kim, Taeseong

AU - Namkoong, Seung

AU - Jang, Ki Hyo

AU - Pyo, Suhkneung

AU - Jang, Seon A.

AU - Sohn, Eun Hwa

PY - 2013/6

Y1 - 2013/6

N2 - Coriolus versicolor has been known to be an immune stimulator effects. For further understanding of the phagocytic activity and the intracellular mechanisms of ß-glucan from C. versicolor (CVG), we examined the phagocytic activity, phosphorylation of Akt and CK2, nucleus translocation of p65 and Ikaros activity in ß-glucan-treated macrophages using RT-PCR, western blotting, and IP assay. The role of Ikaros in regulating phagocytic effects of CVG was also determined using Ikaros dominant negative isoform cells. This study suggests that CK2/Ikaros are positive regulators and novel signaling pathway involved in phagocytosis and contributes to elucidating the mechanism underlying phagocytic activity induced by ß-glucan.

AB - Coriolus versicolor has been known to be an immune stimulator effects. For further understanding of the phagocytic activity and the intracellular mechanisms of ß-glucan from C. versicolor (CVG), we examined the phagocytic activity, phosphorylation of Akt and CK2, nucleus translocation of p65 and Ikaros activity in ß-glucan-treated macrophages using RT-PCR, western blotting, and IP assay. The role of Ikaros in regulating phagocytic effects of CVG was also determined using Ikaros dominant negative isoform cells. This study suggests that CK2/Ikaros are positive regulators and novel signaling pathway involved in phagocytosis and contributes to elucidating the mechanism underlying phagocytic activity induced by ß-glucan.

KW - Beta-glucan

KW - Coriolus versicolor

KW - Dectin-1

KW - Phagocytic activity

KW - RAW264.7

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U2 - 10.1016/j.ijbiomac.2013.03.017

DO - 10.1016/j.ijbiomac.2013.03.017

M3 - Article

C2 - 23500440

AN - SCOPUS:84875494382

SN - 0141-8130

VL - 57

SP - 9

EP - 16

JO - International Journal of Biological Macromolecules

JF - International Journal of Biological Macromolecules

ER -

Effects of ß-glucans from Coriolus versicolor on macrophage phagocytosis are related to the Akt and CK2/Ikaros

Abstract

Keywords

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