Abstract
Batch experiments were conducted to investigate the effect of the nitrate (NO3 −) concentration on the hydrogen production and substrate utilization in a dark fermentation process. The nitrate concentration was varied from 0 to 5,000 mg L−1 in experiments with a mixed culture of anaerobic sludge. Anaerobic sludge was treated with 2 M HCl at pH 2 for the enhanced biohydrogen production. With added NO3 − concentration of 0 g/L, the maximum hydrogen production was 2.2 mol-H2 mol- glucose−1. But, an increase in NO3 − concentration, the hydrogen production decreased to 0.3 from 2.2 mol-H2 mol- glucose−1 at 4,000 mg NO3 −L−1. But, in all conditions, more than 90 % of initially added glucose (10 g L−1) was removed and the microbial community structures were observed similarly in every condition and main germinated species was detected as Clostridium perfringens by DNA cloning. As a result, it was confirmed that the high nitrate concentration at the initial stage, glucose was utilized for nitrate removal reaction than hydrogen production.
Original language | English |
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Pages (from-to) | 27-32 |
Number of pages | 6 |
Journal | Journal of Material Cycles and Waste Management |
Volume | 17 |
Issue number | 1 |
DOIs | |
Publication status | Published - Jan 2013 |
Bibliographical note
Publisher Copyright:© 2013, Springer Japan.
Keywords
- Biohydrogen
- DNA cloning
- FISH (fluorescent in situ hybridization)
- Metabolic pathway
- Nitrate