Effects of nitrate concentration on biohydrogen production and substrate utilization in dark-fermentation

Myoung Joo Lee, Shan Zhang, Yong Beom Cho, Jeong Eun Park, Kwang Hyeon Chang, Sun Jin Hwang

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Batch experiments were conducted to investigate the effect of the nitrate (NO3 ) concentration on the hydrogen production and substrate utilization in a dark fermentation process. The nitrate concentration was varied from 0 to 5,000 mg L−1 in experiments with a mixed culture of anaerobic sludge. Anaerobic sludge was treated with 2 M HCl at pH 2 for the enhanced biohydrogen production. With added NO3 concentration of 0 g/L, the maximum hydrogen production was 2.2 mol-H2 mol- glucose−1. But, an increase in NO3 concentration, the hydrogen production decreased to 0.3 from 2.2 mol-H2 mol- glucose−1 at 4,000 mg NO3 L−1. But, in all conditions, more than 90 % of initially added glucose (10 g L−1) was removed and the microbial community structures were observed similarly in every condition and main germinated species was detected as Clostridium perfringens by DNA cloning. As a result, it was confirmed that the high nitrate concentration at the initial stage, glucose was utilized for nitrate removal reaction than hydrogen production.

Original languageEnglish
Pages (from-to)27-32
Number of pages6
JournalJournal of Material Cycles and Waste Management
Volume17
Issue number1
DOIs
Publication statusPublished - Jan 2013

Bibliographical note

Publisher Copyright:
© 2013, Springer Japan.

Keywords

  • Biohydrogen
  • DNA cloning
  • FISH (fluorescent in situ hybridization)
  • Metabolic pathway
  • Nitrate

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