Effects of Scoparone on differentiation, adhesion, migration, autophagy and mineralization through the osteogenic signalling pathways

Kyung Ran Park, Bomi Kim, Joon Yeop Lee, Ho Jin Moon, Il Keun Kwon, Hyung Mun Yun

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Scoparone (SCOP), an active and efficient coumarin compound derived from Artemisia capillaris Thunb, has been used as a traditional Chinese herbal medicine. Herein, we investigated the effects of SCOP on the osteogenic processes using MC3T3-E1 pre-osteoblasts in in vitro cell systems. SCOP (C11H10O4, > 99.17%) was purified and identified from A. capillaries. SCOP (0.1 to 100 μM concentrations) did not have cytotoxic effects in pre-osteoblasts; however, it promoted alkaline phosphatase (ALP) staining and activity, and mineralized nodule formation under early and late osteogenic induction. SCOP elevated osteogenic signals through the bone morphogenetic protein 2 (BMP2)-Smad1/5/8 pathway, leading to the increased expression of runt-related transcription factor 2 (RUNX2) with its target protein, matrix metallopeptidase 13 (MMP13). SCOP also induced the non-canonical BMP2-MAPKs pathway, but not the Wnt3a-β-catenin pathway. Moreover, SCOP promoted autophagy, migration and adhesion under the osteogenic induction. Overall, the findings of this study demonstrated that SCOP has osteogenic effects associated with cell differentiation, adhesion, migration, autophagy and mineralization.

Original languageEnglish
Pages (from-to)4520-4529
Number of pages10
JournalJournal of Cellular and Molecular Medicine
Volume26
Issue number16
DOIs
Publication statusPublished - Aug 2022

Keywords

  • Artemisia capillaris
  • SCOP
  • autophagy
  • differentiation
  • mineralization
  • osteoblast

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