Effects on lipid peroxidation and antioxidative enzymes of Euonymus alatus in cultured rat hepatocytes

Kyung Woon Kim, Seok Jong Suh, Jong Dae Kim, Soo Sung Kim, In Seon Lee, June Ki Kim, Gyu Tae Chang, Dong Soo Kim, Cheorl Ho Kim

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9 Citations (Scopus)

Abstract

The Euonymus alatus (Thunb.) Sieb. has long been used as a crude drug. In this paper, we investigate the effects of E. alatus on cultured hepatocyte cell system and lipid peroxidation in hydrogen peroxide (H2O2) treatment conditions. The study covers the physiological activity (the antioxidative activity and the nitrite-scavenging effect) of E. alatus. H 2O2 that can produce intracellular free radical was used for inducer of the peroxidation of cellular lipids. Treatment of E. alatus attenuated in cell killing enhanced by increasing concentrations of H 2O2. The increased malondialdehyde level induced by H 2O2 treatment was reduced by pre-treatment of E. alatus. Furthermore, addition of E. alatus in cell culture medium significantly reduced cell killing and content of intracellular antioxidants. Changes in nitrite-scavenging effect of E. alatus at various concentrations (5-25 mg/ml) and various pH levels (pH 1.2, 4.2 and 6.0) were also observed. The present study was also done to investigate the effects of E. alatus on cultured hepatocyte cell system, H2O2-induced cytotoxicity and antioxidative enzyme activities, including catalase, superoxide dismutase, glutathione peroxidase and glutathione S-transferase in H2O 2treatment conditions. E. alatus treatment had significant protective or elevating activities on these antioxidative enzyme activities compared to a normal group. The results indicate that E. alatus provides a strong antioxidant protection of cells against H2O2-induced oxidative stress.

Original languageEnglish
Pages (from-to)60-70
Number of pages11
JournalBasic and Clinical Pharmacology and Toxicology
Volume104
Issue number1
DOIs
Publication statusPublished - Jan 2009

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