Endogenous reference genes and their quantitative real-time PCR assays for genetically modified bread wheat (Triticum aestivum L.) detection

Litao Yang; Sheng Quan; Dabing Zhang

doi:10.1007/978-1-4939-7337-8_16

Endogenous reference genes and their quantitative real-time PCR assays for genetically modified bread wheat (Triticum aestivum L.) detection

Litao Yang, Sheng Quan, Dabing Zhang

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

7 Citations (Scopus)

Abstract

Endogenous reference genes (ERG) and their derivate analytical methods are standard requirements for analysis of genetically modified organisms (GMOs). Development and validation of suitable ERGs is the primary step for establishing assays that monitoring the genetically modified (GM) contents in food/feed samples. Herein, we give a review of the ERGs currently used for GM wheat analysis, such as ACC1, PKABA1, ALMT1, and Waxy-D1, as well as their performances in GM wheat analysis. Also, we discussed one model for developing and validating one ideal RG for one plant species based on our previous research work.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	259-268
Number of pages	10
DOIs	https://doi.org/10.1007/978-1-4939-7337-8_16
Publication status	Published - 2017

Publication series

Name	Methods in Molecular Biology
Volume	1679
ISSN (Print)	1064-3745

Bibliographical note

Publisher Copyright:
© 2017, Springer Science+Business Media LLC.

Keywords

Endogenous reference gene
GMO detection
Genetically modified wheat

Access to Document

10.1007/978-1-4939-7337-8_16

Cite this

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Endogenous reference genes and their quantitative real-time PCR assays for genetically modified bread wheat (Triticum aestivum L.) detection. / Yang, Litao; Quan, Sheng; Zhang, Dabing.
Methods in Molecular Biology. Humana Press Inc., 2017. p. 259-268 (Methods in Molecular Biology; Vol. 1679).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

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Endogenous reference genes and their quantitative real-time PCR assays for genetically modified bread wheat (Triticum aestivum L.) detection

Abstract

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Keywords

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