Enzymatic modification of daidzin using heterologously expressed amylosucrase in Bacillus subtilis

Eun Ryoung Kim, Chan Su Rha, Young Sung Jung, Jung Min Choi, Gi Tae Kim, Dong Hyun Jung, Tae Jip Kim, Dong Ho Seo, Dae Ok Kim, Cheon Seok Park

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

Amylosucrases (ASase, EC 2.4.1.4) from Deinococcus geothermalis (DGAS) and Neisseria polysaccharea (NPAS) were heterologously expressed in Bacillus subtilis. While DGAS was successfully expressed, NPAS was not. Instead, NPAS was expressed in Escherichia coli. Recombinant DGAS and NPAS were purified using nickel-charged affinity chromatography and employed to modify daidzin to enhance its water solubility and bioavailability. Analyses by LC/MS revealed that the major products of transglycosylation using DGAS were daidzein diglucoside and daidzein triglucoside, whereas that obtained by NPAS was only daidzein diglucoside. The optimal bioconversion conditions for daidzein triglucoside, which was predicted to have the highest water-solubility among the daidzin derivatives, was determined to be 4% (w/v) sucrose and 250 mg/L daidzin in sodium phosphate pH 7.0, with a reaction time of 12 h. Taken together, we suggest that the yield and product specificity of isoflavone daidzin transglycosylation may be modulated by the source of ASase and reaction conditions.

Original languageEnglish
Pages (from-to)165-174
Number of pages10
JournalFood Science and Biotechnology
Volume28
Issue number1
DOIs
Publication statusPublished - 11 Feb 2019

Keywords

  • Amylosucrase
  • Bacillus subtilis
  • Daidzin
  • Deinococcus geothermalis
  • Transglycosylation

Fingerprint

Dive into the research topics of 'Enzymatic modification of daidzin using heterologously expressed amylosucrase in Bacillus subtilis'. Together they form a unique fingerprint.

Cite this