Epigallocatechin gallate reduces hypoxia-induced apoptosis in human hepatoma cells

Hae Jeong Park; Dong Hoon Shin; Woo Jin Chung; Kanghyun Leem; Seo Hyun Yoon; Mee Suk Hong; Joo Ho Chung; Jae Hoon Bae; Jae Seok Hwang

doi:10.1016/j.lfs.2005.11.001

Epigallocatechin gallate reduces hypoxia-induced apoptosis in human hepatoma cells

Hae Jeong Park, Dong Hoon Shin, Woo Jin Chung, Kanghyun Leem, Seo Hyun Yoon, Mee Suk Hong, Joo Ho Chung, Jae Hoon Bae, Jae Seok Hwang

Research output: Contribution to journal › Article › peer-review

28 Citations (Scopus)

Abstract

Cell detachment from extracellular matrix is closely related to induction of apoptosis. Epigallocatechin gallate (EGCG) has been shown to have antioxidant effect and to protect hypoxia-induced damage. We investigated whether EGCG reduced hypoxia-induced apoptosis and cell detachment in HepG2 cells. EGCG prevented cell death by hypoxia (0.5% O₂) in a dose-dependent manner (hypoxic cell viability, 54.67%). RT-PCR and caspase3 activity assay showed that the hypoxia-induced cell death was caused by apoptosis increasing mRNA level of BAX, CASP3, and caspase3 activity. EGCG reduced increase of these mRNA and caspase3 activity. Western blot analysis and immunocytochemistry showed that EGCG increased cell adhesion proteins including E-cadherin (CDH1), tumor-associated calcium signal transducer 1 (TACSTD1), and protein tyrosine kinase 2 (PTK2) decreased by hypoxia. Hypoxia-induced apoptosis in HepG2 cells, and EGCG contributed to the HepG2 cell survival by attenuating the apoptosis.

Original language	English
Pages (from-to)	2826-2832
Number of pages	7
Journal	Life Sciences
Volume	78
Issue number	24
DOIs	https://doi.org/10.1016/j.lfs.2005.11.001
Publication status	Published - 8 May 2006

Bibliographical note

Funding Information:
This work was supported by the research promoting grant from the Keimyung University Dongsan Medical Center in 2002.

Keywords

Apoptosis
Epigallocatechin gallate
HepG2 cells
Hypoxia

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10.1016/j.lfs.2005.11.001

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title = "Epigallocatechin gallate reduces hypoxia-induced apoptosis in human hepatoma cells",

abstract = "Cell detachment from extracellular matrix is closely related to induction of apoptosis. Epigallocatechin gallate (EGCG) has been shown to have antioxidant effect and to protect hypoxia-induced damage. We investigated whether EGCG reduced hypoxia-induced apoptosis and cell detachment in HepG2 cells. EGCG prevented cell death by hypoxia (0.5% O2) in a dose-dependent manner (hypoxic cell viability, 54.67%). RT-PCR and caspase3 activity assay showed that the hypoxia-induced cell death was caused by apoptosis increasing mRNA level of BAX, CASP3, and caspase3 activity. EGCG reduced increase of these mRNA and caspase3 activity. Western blot analysis and immunocytochemistry showed that EGCG increased cell adhesion proteins including E-cadherin (CDH1), tumor-associated calcium signal transducer 1 (TACSTD1), and protein tyrosine kinase 2 (PTK2) decreased by hypoxia. Hypoxia-induced apoptosis in HepG2 cells, and EGCG contributed to the HepG2 cell survival by attenuating the apoptosis.",

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author = "Park, {Hae Jeong} and Shin, {Dong Hoon} and Chung, {Woo Jin} and Kanghyun Leem and Yoon, {Seo Hyun} and Hong, {Mee Suk} and Chung, {Joo Ho} and Bae, {Jae Hoon} and Hwang, {Jae Seok}",

note = "Funding Information: This work was supported by the research promoting grant from the Keimyung University Dongsan Medical Center in 2002.",

year = "2006",

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doi = "10.1016/j.lfs.2005.11.001",

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AU - Park, Hae Jeong

AU - Shin, Dong Hoon

AU - Chung, Woo Jin

AU - Leem, Kanghyun

AU - Yoon, Seo Hyun

AU - Hong, Mee Suk

AU - Chung, Joo Ho

AU - Bae, Jae Hoon

AU - Hwang, Jae Seok

N1 - Funding Information: This work was supported by the research promoting grant from the Keimyung University Dongsan Medical Center in 2002.

PY - 2006/5/8

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N2 - Cell detachment from extracellular matrix is closely related to induction of apoptosis. Epigallocatechin gallate (EGCG) has been shown to have antioxidant effect and to protect hypoxia-induced damage. We investigated whether EGCG reduced hypoxia-induced apoptosis and cell detachment in HepG2 cells. EGCG prevented cell death by hypoxia (0.5% O2) in a dose-dependent manner (hypoxic cell viability, 54.67%). RT-PCR and caspase3 activity assay showed that the hypoxia-induced cell death was caused by apoptosis increasing mRNA level of BAX, CASP3, and caspase3 activity. EGCG reduced increase of these mRNA and caspase3 activity. Western blot analysis and immunocytochemistry showed that EGCG increased cell adhesion proteins including E-cadherin (CDH1), tumor-associated calcium signal transducer 1 (TACSTD1), and protein tyrosine kinase 2 (PTK2) decreased by hypoxia. Hypoxia-induced apoptosis in HepG2 cells, and EGCG contributed to the HepG2 cell survival by attenuating the apoptosis.

AB - Cell detachment from extracellular matrix is closely related to induction of apoptosis. Epigallocatechin gallate (EGCG) has been shown to have antioxidant effect and to protect hypoxia-induced damage. We investigated whether EGCG reduced hypoxia-induced apoptosis and cell detachment in HepG2 cells. EGCG prevented cell death by hypoxia (0.5% O2) in a dose-dependent manner (hypoxic cell viability, 54.67%). RT-PCR and caspase3 activity assay showed that the hypoxia-induced cell death was caused by apoptosis increasing mRNA level of BAX, CASP3, and caspase3 activity. EGCG reduced increase of these mRNA and caspase3 activity. Western blot analysis and immunocytochemistry showed that EGCG increased cell adhesion proteins including E-cadherin (CDH1), tumor-associated calcium signal transducer 1 (TACSTD1), and protein tyrosine kinase 2 (PTK2) decreased by hypoxia. Hypoxia-induced apoptosis in HepG2 cells, and EGCG contributed to the HepG2 cell survival by attenuating the apoptosis.

KW - Apoptosis

KW - Epigallocatechin gallate

KW - HepG2 cells

KW - Hypoxia

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Epigallocatechin gallate reduces hypoxia-induced apoptosis in human hepatoma cells

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