Establishing an efficient micropropagation method for Astragalus membranaceus (Huang Qi)

Astragalus membranaceus is known as Hwang-gi in Korea, Huang Qi in Chinese and Radix Astragali in Latin, is a member of Fabaceae family. Traditionally, it has been used as an immunomodulator to treat people with cardiovascular disease as well as colds, fatigue, diarrhea, anorexia, and irregular uterine hemorrhage. In this study, an efficient micropropagation method of A. membranaceus was established, by which micropropagation can be achieved from 2 pathways: direct and indirect. For direct organogenesis, the multi-shoots were induced from apical or lateral shoots in MS medium with BAP at 1.0 mg/L and subsequently, they were proliferated in MS supplemented kinetin at 2.0 mg/L. For indirect organogenesis, the adventitious shoots were regenerated indirectly in MS with BAP at 1.0 mg/L and IBA at 0.5 mg/L media from callus, formed in MS with 2.4-D at 1.0 mg/L. Finally, the single shoot was transferred to 1/2 MS medium for rooting. Regenerated plants grown on hormone-free media for 1 month were transferred to polyethylene pots containing a cocopeat: perlite (3:1; v/v) mixture with a survival rate of approximately 93% 1 month after acclimatization. The results of this study make a significant contribution to the efficient micropropagation of this plant, which is useful for providing abundant and stable resources for further and more in-depth studies of this species such as the extraction of secondary metabolites with potential downstream applications or large-scale micropropagation offers an alternative within shorter time to grow this plant in the field for commercial and pharmaceutical uses.Please confirm if the author names are presented accurately and in the correct sequence (given name, middle name/initial, family name). Author 1 Given name: [Thi Ngoc Anh] Last name [Tran]. Also, kindly confirm the details in the metadata are correct.I confirmed information of all author and correectly in orderPlease provide key message for this article.Whole