Eugenol isolated from the essential oil of Eugenia caryophyllata induces a reactive oxygen species-mediated apoptosis in HL-60 human promyelocytic leukemia cells

Chae Bin Yoo; Ki Tae Han; Kyu Seok Cho; Joohun Ha; Hee Juhn Park; Jung Hwan Nam; Uk Hyun Kil; Kyung Tae Lee

doi:10.1016/j.canlet.2004.11.018

Eugenol isolated from the essential oil of Eugenia caryophyllata induces a reactive oxygen species-mediated apoptosis in HL-60 human promyelocytic leukemia cells

Chae Bin Yoo
, Ki Tae Han
, Kyu Seok Cho
, Joohun Ha
, Hee Juhn Park
, Jung Hwan Nam
, Uk Hyun Kil
, Kyung Tae Lee

Research output: Contribution to journal › Article › peer-review

241 Citations (Scopus)

Abstract

Eugenol is a major component of essential oil isolated from the Eugenia caryophyllata (Myrtaceae), which has been widely used as a herbal drug. In this study, we investigated the effects of eugenol on the cytotoxicity, induction of apoptosis, and the putative pathways of its actions in human promyelocytic leukemia cells (HL-60) under the standard laboratory illumination. Eugenol-treated HL-60 cells displayed features of apoptosis including DNA fragmentation and formation of DNA ladders in agarose gel electrophoresis. We observed that eugenol transduced the apoptotic signal via ROS generation, thereby inducing mitochondrial permeability transition (MPT), reducing anti-apoptotic protein bcl-2 level, inducing cytochrome c release to the cytosol, and subsequent apoptotic cell death. Taken together, the present study demonstrated that ROS plays a critical role in eugenol-induced apoptosis in HL-60, and this is the first report on the mechanism of the anticancer effect of eugenol.

Original language	English
Pages (from-to)	41-52
Number of pages	12
Journal	Cancer Letters
Volume	225
Issue number	1
DOIs	https://doi.org/10.1016/j.canlet.2004.11.018
Publication status	Published - 8 Jul 2005

Bibliographical note

Funding Information:
This work was supported by a grant No. R13-2002-020-01002-0 from the Korea Science and Engineering Foundation.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Apoptosis
Eugenol
Glutathione
Mitochondrial permeability transition
ROS

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10.1016/j.canlet.2004.11.018

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title = "Eugenol isolated from the essential oil of Eugenia caryophyllata induces a reactive oxygen species-mediated apoptosis in HL-60 human promyelocytic leukemia cells",

abstract = "Eugenol is a major component of essential oil isolated from the Eugenia caryophyllata (Myrtaceae), which has been widely used as a herbal drug. In this study, we investigated the effects of eugenol on the cytotoxicity, induction of apoptosis, and the putative pathways of its actions in human promyelocytic leukemia cells (HL-60) under the standard laboratory illumination. Eugenol-treated HL-60 cells displayed features of apoptosis including DNA fragmentation and formation of DNA ladders in agarose gel electrophoresis. We observed that eugenol transduced the apoptotic signal via ROS generation, thereby inducing mitochondrial permeability transition (MPT), reducing anti-apoptotic protein bcl-2 level, inducing cytochrome c release to the cytosol, and subsequent apoptotic cell death. Taken together, the present study demonstrated that ROS plays a critical role in eugenol-induced apoptosis in HL-60, and this is the first report on the mechanism of the anticancer effect of eugenol.",

keywords = "Apoptosis, Eugenol, Glutathione, Mitochondrial permeability transition, ROS",

author = "Yoo, \{Chae Bin\} and Han, \{Ki Tae\} and Cho, \{Kyu Seok\} and Joohun Ha and Park, \{Hee Juhn\} and Nam, \{Jung Hwan\} and Kil, \{Uk Hyun\} and Lee, \{Kyung Tae\}",

note = "Funding Information: This work was supported by a grant No. R13-2002-020-01002-0 from the Korea Science and Engineering Foundation.",

year = "2005",

month = jul,

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doi = "10.1016/j.canlet.2004.11.018",

language = "English",

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T1 - Eugenol isolated from the essential oil of Eugenia caryophyllata induces a reactive oxygen species-mediated apoptosis in HL-60 human promyelocytic leukemia cells

AU - Yoo, Chae Bin

AU - Han, Ki Tae

AU - Cho, Kyu Seok

AU - Ha, Joohun

AU - Park, Hee Juhn

AU - Nam, Jung Hwan

AU - Kil, Uk Hyun

AU - Lee, Kyung Tae

N1 - Funding Information: This work was supported by a grant No. R13-2002-020-01002-0 from the Korea Science and Engineering Foundation.

PY - 2005/7/8

Y1 - 2005/7/8

N2 - Eugenol is a major component of essential oil isolated from the Eugenia caryophyllata (Myrtaceae), which has been widely used as a herbal drug. In this study, we investigated the effects of eugenol on the cytotoxicity, induction of apoptosis, and the putative pathways of its actions in human promyelocytic leukemia cells (HL-60) under the standard laboratory illumination. Eugenol-treated HL-60 cells displayed features of apoptosis including DNA fragmentation and formation of DNA ladders in agarose gel electrophoresis. We observed that eugenol transduced the apoptotic signal via ROS generation, thereby inducing mitochondrial permeability transition (MPT), reducing anti-apoptotic protein bcl-2 level, inducing cytochrome c release to the cytosol, and subsequent apoptotic cell death. Taken together, the present study demonstrated that ROS plays a critical role in eugenol-induced apoptosis in HL-60, and this is the first report on the mechanism of the anticancer effect of eugenol.

AB - Eugenol is a major component of essential oil isolated from the Eugenia caryophyllata (Myrtaceae), which has been widely used as a herbal drug. In this study, we investigated the effects of eugenol on the cytotoxicity, induction of apoptosis, and the putative pathways of its actions in human promyelocytic leukemia cells (HL-60) under the standard laboratory illumination. Eugenol-treated HL-60 cells displayed features of apoptosis including DNA fragmentation and formation of DNA ladders in agarose gel electrophoresis. We observed that eugenol transduced the apoptotic signal via ROS generation, thereby inducing mitochondrial permeability transition (MPT), reducing anti-apoptotic protein bcl-2 level, inducing cytochrome c release to the cytosol, and subsequent apoptotic cell death. Taken together, the present study demonstrated that ROS plays a critical role in eugenol-induced apoptosis in HL-60, and this is the first report on the mechanism of the anticancer effect of eugenol.

KW - Apoptosis

KW - Eugenol

KW - Glutathione

KW - Mitochondrial permeability transition

KW - ROS

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DO - 10.1016/j.canlet.2004.11.018

M3 - Article

C2 - 15922856

AN - SCOPUS:19544362882

SN - 0304-3835

VL - 225

SP - 41

EP - 52

JO - Cancer Letters

JF - Cancer Letters

IS - 1

ER -

Eugenol isolated from the essential oil of Eugenia caryophyllata induces a reactive oxygen species-mediated apoptosis in HL-60 human promyelocytic leukemia cells

Abstract

Bibliographical note

UN SDGs

Keywords

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